The role of extracellular vesicles from stored RBC units in B lymphocyte survival and plasma cell differentiation

被引：14
|
作者：
Gao, Yuhan [1 ]
Jin, Haiqiang [2 ]
Tan, Hui [3 ]
Wang, Yan [4 ,5 ]
Wu, Jia [4 ,5 ]
Wang, Yuqing [4 ,5 ]
Zhang, Jianhua [1 ]
Yang, Ying [1 ]
Tian, Wenqin [1 ]
Hou, Ruiqin [1 ]
机构：
[1] Peking Univ, Dept Blood Transfus, Peoples Hosp, Xizhimen South Rd 11, Beijing 100035, Peoples R China
[2] Peking Univ, Dept Neurol, Hosp 1, Beijing, Peoples R China
[3] Shenzhen Univ, Guangdong Innovat Platform Translat Res Cerebrov, Affiliated Hosp 1, Shenzhen, Peoples R China
[4] Peking Univ, Minist Publ Hlth, Hlth Sci Ctr, Dept Immunol, Beijing, Peoples R China
[5] Peking Univ, Minist Publ Hlth, Hlth Sci Ctr, Key Lab Med Immunol, Beijing, Peoples R China
来源：
JOURNAL OF LEUKOCYTE BIOLOGY | 2020年 / 108卷 / 06期
基金：
中国国家自然科学基金;
关键词：
extracellular vesicle; B cell; plasma cell; antibody production; RED-BLOOD-CELLS; FAS LIGAND MOLECULES; HLA CLASS-I; FLOW-CYTOMETRY; TRANSFUSION; MICROPARTICLES; STORAGE; MECHANISMS; IMMUNOMODULATION; INFLAMMATION;
D O I：
10.1002/JLB.1A0220-666R
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Extracellular vesicles (EVs) are small, double-membrane vesicles derived from erythrocytes, leukocytes, platelets, and cells of multiple tissues under physiologic or pathologic conditions. The role of EVs in stored RBC units is of great interest with respect to transfusion-related immunomodulation. The current study focuses on the quantity of EVs isolated from stored RBC units and their action on B cell-mediated immune responses. The in vitro experiment demonstrated that EVs exhibited a negative role in B cell survival, plasmacytic differentiation, and class switch recombination under LPS stimulation. Furthermore, LPS-induced antibody production was significantly decreased after EVs injection in vivo. Biochemical analysis revealed that EVs hampered the expression of Blimp-1 and IRF4 and the activation of NF-kappa B pathway in LPS-primed B cells. Overall, these data imply a vital role for EVs isolated from RBC units in B cell-mediated immune responses.
引用
收藏
页码：1765 / 1776
页数：12
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