Thermostable amidase catalyzed production of isonicotinic acid from isonicotinamide

被引：7
|
作者：
Mehta, Praveen Kumar [1 ,2 ]
Bhatia, Shashi Kant [1 ,3 ]
Bhatia, Ravi Kant [1 ]
Bhalla, Tek Chand [1 ]
机构：
[1] Himachal Pradesh Univ, Dept Biotechnol, Shimla 171005, Himachal Prades, India
[2] Univ Fed Sergipe, Lab Anal Cromatograf & Flavour LAF, BR-49100000 Sao Cristovao, SE, Brazil
[3] Konkuk Univ, Dept Microbial Engn, Seoul 143701, South Korea
来源：
PROCESS BIOCHEMISTRY | 2015年 / 50卷 / 09期
关键词：
Geobacillus subterraneus; Thermostable; Amidase; Isonicotinamide; Isonicotinic acid; Fed batch; ACYL TRANSFER ACTIVITY; ASPERGILLUS-NIGER K10; BENCH-SCALE; PSEUDOMONAS-PUTIDA; MILD HYDROLYSIS; NITRILASE; BIOTRANSFORMATION; ENZYMES; 4-CYANOPYRIDINE;
D O I：
10.1016/j.procbio.2015.05.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The biotransformation of isonicotinamide was investigated using thermophilic intracellular amidase produced from Geobacillus subterraneus RL-2a. Various process parameters, including amount of biocatalyst, substrate feeding rate, enzyme-to-substrate ratio and operational thermostability were systematically examined with the aim of achieving complete substrate conversion and high productivity. In 1 L fed batch reaction containing 0.1 M isonicotinamide, in 0.2 M potassium phosphate buffer (pH 6.5, 200 rpm) and 8 U ml(-1) amidase activity (12.48 mg dcw ml(-1)) of whole cells of G. subterraneus RL-2a (as biocatalyst) resulted in a yield of 0.1 M of isonicotinic acid after 50 min reaction time at 70 degrees C and a total of 61.55 g isonicotinic acid was produced at a rate of 1.18 g h(-1) g(-1) dcw respectively. The volumetric productivity was 14.8 g h(-1)l(-1). (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1400 / 1404
页数：5
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