Long Noncoding RNA LINC00634 Functions as an Oncogene in Esophageal Squamous Cell Carcinoma Through the miR-342-3p/Bcl2L1 Axis

被引：12

作者：

Zhang, Xiaohong ^{[1
]}

Feng, Yinman ^{[1
]}

Gao, Yanli ^{[1
]}

Hu, Jun ^{[1
]}

机构：

[1] Zhengzhou Hlth Vocat Coll, 84 Middle Hanghai Rd, Zhengzhou 450005, Henan, Peoples R China

来源：

TECHNOLOGY IN CANCER RESEARCH & TREATMENT | 2020年 / 19卷

关键词：

esophageal cancer; lncRNA; miR-342-3p; PROLIFERATION; ANTISENSE; CANCER; TRANSCRIPTION; PROGRESSION; METASTASIS; EXPRESSION; LANDSCAPE; MIGRATION;

D O I：

10.1177/1533033820928508

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Many long noncoding RNAs reportedly have tumor suppressive roles or are oncogenic in esophageal cancer. We have previously performed a chip-based expression analysis of primary esophageal cancer tissues and found that the expression of LINC00634 in these tissues was higher than that in nontumor tissues. Quantitative real-time-polymerase chain reaction, cell counting kit-8, flow cytometry, caspase3/7 assay, dual-luciferase reporter assay, and restore assay were used to detect the proliferative and apoptotic effects of LINC00634 in esophageal cancer cells. The results showed that the expression of LINC00634 in these tissues was higher than that in nontumor tissues and associated with tumor-node-metastasis (TNM) stage of patients. Knockdown of LINC00634 decreased cell viability and increased cell apoptosis levels in EC9706 and EC1 cells. LINC00634 could target Bcl2L1 through miR-342-3p. In this study, we show that LINC00634 is upregulated in esophageal cancer. We also show that the knockdown of LINC00634 decreased cell viability and increased cell apoptosis levels in EC9706 and EC1 cells through the miR-342-3p/Bcl2L1 axis.

引用

页数：7

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