Antigenic profiles of recombinant proteins from Mycobacterium avium subsp paratuberculosis in sheep with Johne's disease

被引：13
作者：
Bannantine, John P. [2 ]
Rosu, Valentina [1 ]
Zanetti, Stefania [1 ,3 ]
Rocca, Stefano [4 ]
Ahmed, Niyaz [3 ,5 ]
Sechi, Leonardo A. [1 ,3 ]
机构：
[1] Univ Sassari, Sez Microbiol Sperimentale & Clin, Dipartimento Sci Biomed, I-07100 Sassari, Italy
[2] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA
[3] ISOGEM Collaborat Network Tubercle Bacilli, Sassari, Italy
[4] Univ Sassari, Ist Patol Gen Anat Patol & Clin Ostetrico Chirurg, I-07100 Sassari, Italy
[5] Ctr DNA Fingerprinting & Diagnost, Pathogen Evolut Grp, Hyderabad, Andhra Pradesh, India
来源：
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2008年 / 122卷 / 1-2期
关键词：
Mycobacterium avium subsp paratuberculosis; Johne's disease; sheep; antigens;
D O I：
10.1016/j.vetimm.2007.10.020
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Methods to improve the ELISA test to detect Mycobacterium avium subsp. paratuberculosis have been explored over several years. Previously, selected recombinant proteins of M. avium subspecies paratuberculosis were found to be immunogenic in cattle with Johne's disease. In the present study, antibody responses of infected and healthy sheep were evaluated using 18 purified recombinant proteins in an ELISA-based format for the serodiagnosis of ovine paratuberculosis. These selected recombinant proteins represent heat shock proteins, hypothetical proteins and cell surface proteins of M. avium subsp. paratuberculosis. Whereas, Map0862 (a gene uniquely present in M. avium subspecies paratuberculosis) and Map3786 encoded protein solicited the strongest antibody response in infected sheep. The protein encoded by Map2116c showed the weakest antibody response among the animals tested. Although none of the recombinant proteins detected all 11 infected sheep singly, antibodies to Map0862 were detected in 9 of 11 (81%) infected sheep. Furthermore, ovine responses to these selected antigens were assessed temporally over the course of 1 year during which we found a spiking effect rather than an incremental increase of antibody reactivity. This study evaluated multiple M. avium subsp. paratuberculosis recombinant proteins in an ELISA-based format for sheep. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：116 / 125
页数：10
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