Response of the photosynthetic apparatus to UV-A and red light in the phytochrome B-deficient Arabidopsis thaliana L. hy3 mutant

被引：19

作者：

Kreslavski, V. D. ^{[1
,2
]}

Schmitt, F. -J. ^{[3
]}

Keuer, C. ^{[3
]}

Friedrich, T. ^{[3
]}

Shirshikova, G. N. ^{[1
]}

Zharmukhamedov, S. K. ^{[1
]}

Kosobryukhov, A. A. ^{[1
]}

Allakhverdiev, S. I. ^{[1
,2
,4
]}

机构：

[1] Russian Acad Sci, Inst Basic Biol Problems, Inst Skaya St 2, Pushchino 142290, Moscow Region, Russia

[2] Russian Acad Sci, Inst Plant Physiol, Controlled Photobiosynth Lab, Botanicheskaya St 35, Moscow 127276, Russia

[3] Tech Univ Berlin, Inst Chem Sekr PC 14, Max Volmer Lab Biophys Chem, Str 17 Juni 135, D-10623 Berlin, Germany

[4] Moscow MV Lomonosov State Univ, Dept Plant Physiol, Fac Biol, Leninskie Gory 1-12, Moscow 119991, Russia

来源：

PHOTOSYNTHETICA | 2016年 / 54卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

Arabidopsis thaliana; chlorophyll a fluorescence; photosystem II; phytochrome system; stress resistance; transcription; ultraviolet; PHOTOSYSTEM-II; CHLOROPHYLL FLUORESCENCE; INCREASES RESISTANCE; ENVIRONMENTAL-STRESS; WHEAT LEAVES; RT-PCR; PLANTS; RADIATION; DAMAGE; GENE;

D O I：

10.1007/s11099-016-0212-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The effect of UV-A radiation (365 nm) and the protective effect of preillumination with red light (RL, 664 nm, 10 min) or with a combination of red and far-red light (FRL, 727 nm, 10 min) on the activity of the PSII as well as the expression levels of selected genes, especially those encoding chloroplast proteins (sAPX, tAPX, CAB1, and D1), were studied in leaves of the 26-d-old hy3 mutant of Arabidopsis thaliana, which is deficient in the phytochrome B apoprotein. The effects were compared with corresponding effects observed in the hy2 mutant of A. thaliana, which is deficient in the phytochrome chromophore. Illumination with UV-A decreased the photosynthetic pigment content, the maximum photochemical quantum yield of PSII (F-v/F-m), and the effective quantum yield of PSII (I broken vertical bar(PSII)). The reduction of the F-v/F-m ratio and I broken vertical bar(PSII) was more pronounced in the mutants as compared to wild-type plants (WT). The preillumination of the leaves with RL caused a significant reduction in the inhibitory effect of UV-radiation on the PSII activity in the WT plants, but it caused only a small decrease in the hy3 mutant. The preillumination of leaves with RL and FRL combination compensated the protective effect of RL on the UV-induced decrease of the fluorescence parameters in the WT. Such reversibility is typical for involvement of red/far-red reversible phytochromes at low intensity light. The results suggest an important role of red/far-red reversible phytochromes (phytochrome B) in the resistance of PSII to UV-A radiation caused by changes in contents of either carotenoids or other UV-absorbing pigments probably through biosynthesis of these pigments. The data also demonstrated that phytochrome B and other phytochromes can affect the PSII stress resistance by the fast regulation of the expression of genes encoding antioxidant enzymes and transcription factors at the step of gene transcription.

引用

页码：321 / 330

页数：10

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