Blue-Red LED wavelength shifting strategy for enhancing beta-carotene production from halotolerant microalga, Dunaliella salina

被引:49
作者
Han, Sang-Il [1 ]
Kim, Sok [1 ]
Lee, Changsu [2 ]
Choi, Yoon-E [1 ]
机构
[1] Korea Univ, Div Environm Sci & Ecol Engn, Seoul 02841, South Korea
[2] World Inst Kimchi, Microbiol & Funct Res Grp, Gwangju 61755, South Korea
基金
新加坡国家研究基金会;
关键词
Dunaliella salina; beta-carotene; light-emitting diode; wavelength shift; adaptive laboratory evolution; FATTY-ACID-METABOLISM; ENHANCEMENT;
D O I
10.1007/s12275-019-8420-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In the present study, to improve the photosynthetic betacarotene productivity of Dunaliella salina, a blue-red LED wavelength-shifting system (B-R system) was investigated. Dunaliella salina under the B-R system showed enhanced density and beta-carotene productivity compared to D. salina cultivated under single light-emitting diode light wavelengths (blue, white, and red light-emitting diode). Additionally, we developed blue light-adapted D. salina (ALE-D. salina) using an adaptive laboratory evolution (ALE) approach. In combination with the B-R system applied to ALE-D. salina (ALE B-R system), the beta-carotene concentration (33.94 +/- 0.52 M) was enhanced by 19.7% compared to that observed for the non-ALE-treated wild-type of D. salina (intact D. salina) under the B-R system (28.34 +/- 0.24 M).
引用
收藏
页码:101 / 106
页数:6
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