GmLecRlk, a Lectin Receptor-like Protein Kinase, Contributes to Salt Stress Tolerance by Regulating Salt-Responsive Genes in Soybean

被引:20
|
作者
Zhang, Yanzheng [1 ]
Fang, Qingwei [1 ]
Zheng, Jiqiang [1 ]
Li, Zeyang [1 ]
Li, Yue [1 ]
Feng, Yuan [1 ]
Han, Yingpeng [1 ]
Li, Yongguang [1 ]
机构
[1] Northeast Agr Univ, Minist Agr & Rural Affairs, Minist Educ China, Key Lab Soybean Biol,Key Lab Soybean Biol & Breed, Harbin 150030, Peoples R China
关键词
soybean; GmLecRlk; lectin receptor protein kinase; salt tolerance; RNA-seq; LEUCINE-RICH REPEAT; ARABIDOPSIS; RECOGNITION; DROUGHT;
D O I
10.3390/ijms23031030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Soybean [Glycine max (L.) Merr.] is an important oil crop that provides valuable resources for human consumption, animal feed, and biofuel. Through the transcriptome analysis in our previous study, GmLecRlk (Glyma.07G005700) was identified as a salt-responsive candidate gene in soybean. In this study, qRT-PCR analysis showed that the GmLecRlk gene expression level was significantly induced by salt stress and highly expressed in soybean roots. The pCAMBIA3300-GmLecRlk construct was generated and introduced into the soybean genome by Agrobacterium rhizogenes. Compared with the wild type (WT), GmLecRlk overexpressing (GmLecRlk-ox) soybean lines had significantly enhanced fresh weight, proline (Pro) content, and catalase (CAT) activity, and reduced malondialdehyde (MDA) and H2O2 content under salt stress. These results show that GmLecRlk gene enhanced ROS scavenging ability in response to salt stress in soybean. Meanwhile, we demonstrated that GmLecRlk gene also conferred soybean salt tolerance when it was overexpressed alone in soybean hairy root. Furthermore, the combination of RNA-seq and qRT-PCR analysis was used to determine that GmLecRlk improves the salt tolerance of soybean by upregulating GmERF3, GmbHLH30, and GmDREB2 and downregulating GmGH3.6, GmPUB8, and GmLAMP1. Our research reveals a new mechanism of salt resistance in soybean, which exposes a novel avenue for the cultivation of salt-resistant varieties.
引用
收藏
页数:13
相关论文
共 50 条
  • [11] Soybean receptor-like protein kinase genes: Paralogous divergence of a gene family
    Yamamoto, E
    Knap, HT
    MOLECULAR BIOLOGY AND EVOLUTION, 2001, 18 (08) : 1522 - 1531
  • [12] Arabidopsis nucleoporin NUP96 mediates plant salt tolerance by modulating the transcription of salt-responsive genes
    Xiaomin Yang
    Chengcheng Ji
    Xinxin Liu
    Zhaoxin Wei
    Qiuying Pang
    Aiqin Zhang
    Planta, 2024, 259
  • [13] Duplication and diversification of lectin receptor-like kinases (LecRLK) genes in soybean
    Liu, Ping-Li
    Huang, Yuan
    Shi, Peng-Hao
    Yu, Meng
    Xie, Jian-Bo
    Xie, LuLu
    SCIENTIFIC REPORTS, 2018, 8
  • [14] Duplication and diversification of lectin receptor-like kinases (LecRLK) genes in soybean
    Ping-Li Liu
    Yuan Huang
    Peng-Hao Shi
    Meng Yu
    Jian-Bo Xie
    LuLu Xie
    Scientific Reports, 8
  • [15] Arabidopsis nucleoporin NUP96 mediates plant salt tolerance by modulating the transcription of salt-responsive genes
    Yang, Xiaomin
    Ji, Chengcheng
    Liu, Xinxin
    Wei, Zhaoxin
    Pang, Qiuying
    Zhang, Aiqin
    PLANTA, 2024, 259 (02)
  • [16] LecRK-VII.1, a Lectin Receptor-Like Kinase, Mediates the Regulation of Salt Stress and Jasmonic Acid Response in Arabidopsis
    Cheng Zhang
    Jinyan Li
    Xinhong Guo
    Baode Zhu
    Wenjun Xiao
    Ping Wang
    Miao Jiang
    Shuai Hu
    Xiutao Lu
    Zhuang He
    Ping Chen
    Journal of Plant Growth Regulation, 2017, 36 : 385 - 401
  • [17] LecRK-VII.1, a Lectin Receptor-Like Kinase, Mediates the Regulation of Salt Stress and Jasmonic Acid Response in Arabidopsis
    Zhang, Cheng
    Li, Jinyan
    Guo, Xinhong
    Zhu, Baode
    Xiao, Wenjun
    Wang, Ping
    Jiang, Miao
    Hu, Shuai
    Lu, Xiutao
    He, Zhuang
    Chen, Ping
    JOURNAL OF PLANT GROWTH REGULATION, 2017, 36 (02) : 385 - 401
  • [18] GsCBRLK, a calcium/calmodulin-binding receptor-like kinase, is a positive regulator of plant tolerance to salt and ABA stress
    Yang, Liang
    Ji, Wei
    Zhu, Yanming
    Gao, Peng
    Li, Yong
    Cai, Hua
    Bai, Xi
    Guo, Dianjing
    JOURNAL OF EXPERIMENTAL BOTANY, 2010, 61 (09) : 2519 - 2533
  • [19] Receptor-like kinase OsSIK1 improves drought and salt stress tolerance in rice (Oryza sativa) plants
    Ouyang, Shou-Qiang
    Liu, Yun-Feng
    Liu, Peng
    Lei, Gang
    He, Si-Jie
    Ma, Biao
    Zhang, Wan-Ke
    Zhang, Jin-Song
    Chen, Shou-Yi
    PLANT JOURNAL, 2010, 62 (02): : 316 - 329
  • [20] A leucine-rich repeat receptor-like kinase, OsSTLK, modulates salt tolerance in rice
    Lin, Faming
    Li, Shen
    Wang, Ke
    Tian, Haoran
    Gao, Junfeng
    Zhao, Quanzhi
    Du, Changqing
    PLANT SCIENCE, 2020, 296