Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment

被引：10

作者：

Fofana, Aminata ^{[1
,2
]}

Gendrin, Mathilde ^{[3
,4
]}

Romoli, Ottavia ^{[3
,4
]}

Yarbanga, G. Armel Bienvenu ^{[1
]}

Ouedraogo, Georges Anicet ^{[2
]}

Yerbanga, Rakiswende Serge ^{[1
,5
]}

Ouedraogo, Jean-Bosco ^{[1
,5
]}

机构：

[1] Inst Rech Sci Sante, Bobo Dioulasso, Burkina Faso

[2] Univ Nazi Boni, Bobo Dioulasso 1091, Burkina Faso

[3] Inst Pasteur, Microbiota Insect Vectors Grp, Cayenne 97306, French Guiana

[4] Univ Paris, Dept Insect Vectors, Inst Pasteur, F-75015 Paris, France

[5] Inst Sci & Tech, Bobo Dioulasso 2779, Burkina Faso

来源：

ISCIENCE | 2021年 / 24卷 / 12期

基金：

欧盟地平线“2020”;

关键词：

Entomology; Microbiome;

D O I：

10.1016/j.isci.2021.103416

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The microbiota of Anopheles mosquitoes influences malaria transmission. Antibiotics ingested during a blood mead impact the mosquito microbiome and malaria transmission, with substantial differences between drugs. Here, we assessed if amoxicillin affects the gut mosquito microbiota. We collected Anopheles larvae in Burkina Faso, kept them in semi-field conditions, and offered a blood meal to adult females. We tested the impact of blood supplementation with two alternative amoxicillin preparations on microbiota composition, determined by high-throughput sequencing in individual gut samples. Our analysis detected four major genera, Eliza ethkingia, Wigglesworthia, saia, and Serratia. The anti-biotic treatment significantly affected overall microbiota composition, with a specific decrease in the relative abundance of Eliza ethkingia and A saia during blood digestion. Besides its interest on the influence of amoxicillin on the mosquito microbiota, our study proposes a thorough approach to report negative-control data of high-throughput sequencing studies on samples with a reduced microbial load.

引用

页数：22

共 51 条

[1] A retrospective audit of antibiotic prescriptions in primary health-care facilities in Eastern Region, Ghana [J].

Ahiabu, Mary-Anne ;

Tersbol, Britt P. ;

Biritwum, Richard ;

Bygbjerg, Ib C. ;

Magnussen, Pascal .

HEALTH POLICY AND PLANNING, 2016, 31 (02) :250-258

[2]

Base Nationale de Donnees Topographiques du Burkina Faso (BNDT), 2015, CART BURK FAS AL KAR

[3]

Base Nationale de Donnees Topographiques du Burkina Faso (BNDT), 2015, CART DISTR BURK FAS

[4] Deep Sequencing Analyses of Low Density Microbial Communities: Working at the Boundary of Accurate Microbiota Detection [J].

Biesbroek, Giske ;

Sanders, Elisabeth A. M. ;

Roeselers, Guus ;

Wang, Xinhui ;

Caspers, Martien P. M. ;

Trzcinski, Krzysztof ;

Bogaert, Debby ;

Keijser, Bart J. F. .

PLOS ONE, 2012, 7 (03)

[5] Midgut Microbiota of the Malaria Mosquito Vector Anopheles gambiae and Interactions with Plasmodium falciparum Infection [J].

Boissiere, Anne ;

Tchioffo, Majoline T. ;

Bachar, Dipankar ;

Abate, Luc ;

Marie, Alexandra ;

Nsango, Sandrine E. ;

Shahbazkia, Hamid R. ;

Awono-Ambene, Parfait H. ;

Levashina, Elena A. ;

Christen, Richard ;

Morlais, Isabelle .

PLOS PATHOGENS, 2012, 8 (05)

[6] Trimmomatic: a flexible trimmer for Illumina sequence data [J].

Bolger, Anthony M. ;

Lohse, Marc ;

Usadel, Bjoern .

BIOINFORMATICS, 2014, 30 (15) :2114-2120

[7]

Callahan BJ, 2016, NAT METHODS, V13, P581, DOI [10.1038/NMETH.3869, 10.1038/nmeth.3869]

[8] Microbial Diversity of Adult Aedes aegypti and Water Collected from Different Mosquito Aquatic Habitats in Puerto Rico [J].

Caragata, E. P. ;

Otero, L. M. ;

Tikhe, C. V. ;

Barrera, R. ;

Dimopoulos, G. .

MICROBIAL ECOLOGY, 2022, 83 (01) :182-201

[9] Gut bacteria differentially affect egg production in the anautogenous mosquito Aedes aegypti and facultatively autogenous mosquito Aedes atropalpus (Diptera: Culicidae) [J].

Coon, Kerri L. ;

Brown, Mark R. ;

Strand, Michael R. .

PARASITES & VECTORS, 2016, 9

[10] Considerations for mosquito microbiome research from the Mosquito Microbiome Consortium [J].

Dada, Nsa ;

Jupatanakul, Natapong ;

Minard, Guillaume ;

Short, Sarah M. ;

Akorli, Jewelna ;

Villegas, Luis Martinez .

MICROBIOME, 2021, 9 (01)

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