Stimulated Release of Cholesterol from Liposomal Membranes by a PEGylated Phospholipid

被引：4

作者：

Janout, Vaclav ^{[1
]}

Daly, Trevor A. ^{[1
]}

Cline, Lauren L. ^{[1
]}

Kulp, Leah J. ^{[1
]}

Regen, Steven L. ^{[1
]}

机构：

[1] Lehigh Univ, Dept Chem, Bethlehem, PA 18015 USA

来源：

BIOCONJUGATE CHEMISTRY | 2012年 / 23卷 / 03期

关键词：

UNILAMELLAR VESICLES; LIVING CELLS; DEHYDROERGOSTEROL; FLUORESCENCE; HOMEOSTASIS; EXCHANGE;

D O I：

10.1021/bc200669e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

PEGylated phospholipids are commonly used to increase the blood-circulation time of liposomes by providing a steric barrier around them. This paper documents a fundamentally new property of these lipids-an ability to stimulate the release of cholesterol from phospholipid membranes. Evidence for such stimulation has been obtained by measuring the transport of dehydroergosterol (DHE), a fluorescent simulant of cholesterol, from donor liposomes made from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000 (DSPE-PEG(2000)), and DHE to acceptor liposomes made from POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), and cholesterol. The potential of PEGylated lipids to serve as novel cholesterol-lowering agents is briefly discussed.

引用

页码：336 / 339

页数：4

共 19 条

[1] Kinetics and thermodynamics of the association of dehydroergosterol with lipid bilayer membranes [J].