Micropropagation of Cattleya: Improved In Vitro Rooting and Acclimatization

被引：13
|
作者：
Dewir, Yaser Hassan [1 ,2 ]
El-Mahrouk, Mohammed Elsayed [2 ]
Murthy, Hosakatte Niranjana [3 ]
Paek, Kee Yoeup [4 ]
机构：
[1] King Saud Univ, Coll Food & Agr Sci, Plant Prod Dept, Riyadh 11451, Saudi Arabia
[2] Kafrelsheikh Univ, Fac Agr, Dept Hort, Kafr Al Sheikh 33516, Egypt
[3] Karnatak Univ, Dept Bot, Dharwad 580003, Karnataka, India
[4] Chungbuk Natl Univ, Res Ctr Dev Adv Hort Technol, Cheongju 361763, South Korea
来源：
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY | 2015年 / 56卷 / 01期
关键词：
auxins; growing substrates; light irradiance; root induction; tissue culture; PROPAGATION;
D O I：
10.1007/s13580-015-0108-z
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
The present study reports optimization of in vitro rooting of Cattleya shoots with the aim to increase survival rate during acclimatization Shoots (2.0 - 2.5 cm) which were regenerated in vitro were cultured on Murashige and Skoog (MS) medium and the effect of various parameters such as type and concentration of auxin [0.3, 0.6, 1.2 and 2.4 mg.L-1 indole butyric acid (IBA) and naphthaleneacetic acid (NAA)], medium strength (full and half strength), sucrose concentration (0, 15 and 30 g.L-1:1) and light intensity [photosynthetic photon flux density (PHD) of 30, 60, 90 mu mol.m(-2).s(-1) were tested. Half strength MS medium supplemented with 2.4 mg.L-1 IBA, 3% sucrose and 60 mu mol.m(-2).s(-1) PPM were found as suitable conditions for optimal induction of roots. In vitro regenerated Cattleya plantlets were successfully transferred to potting medium containing bark:perlite:peatmoss (1:1:1) and perlite:peatmoss (1: 1), and 98.3 and 80.7% were survived respectively.
引用
收藏
页码：89 / 93
页数：5
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