Large-scale preparation of the oligosaccharide phosphate fraction of Pichia holstii NRRL Y-2448 phosphomannan for use in the manufacture of PI-88

被引：56
作者：
Ferro, V [1 ]
Fewings, K [1 ]
Palermo, MC [1 ]
Li, CP [1 ]
机构：
[1] Progen Ind Ltd, Dept Res & Dev, Richlands BC, Qld 4077, Australia
来源：
CARBOHYDRATE RESEARCH | 2001年 / 332卷 / 02期
关键词：
Pichia holstii; phosphomannan; oligosaccharide phosphate fraction; PI-88;
D O I：
10.1016/S0008-6215(01)00061-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mild acid-catalysed hydrolysis of the extracellular phosphomannan of the yeast Pichia holstii NRRL Y-2448 produces a high-molecular-weight phosphomannan core, a low-molecular-weight oligosaccharide phosphate fraction, and a neutral oligosaccharide fraction. A method was developed for the large-scale preparation of the oligosaccharide phosphate fraction, consisting predominantly of the pentasaccharide phosphate, 6-O-PO3H2-alpha -D-Man-(1 --> 3)-alpha -D-Man-(1 --> 3)-alpha -D-Man-(1 --> 3)-alpha -D-Man-(1 --> 2)-D-Man, for use in the manufacture of the promising new anti-cancer agent, PI-88. Further insights were also gained into the structure of the phosphomannan by HPLC analysis of the time course of the hydrolysis reaction. (C) 2001 Elsevier Science Ltd. All rights reserved.
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收藏
页码：183 / 189
页数：7
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