Anti-ADAMTS13 autoantibodies in immune-mediated thrombotic thrombocytopenic purpura do not hamper ELISA-based quantification of ADAMTS13 antigen

被引：17

作者：

Dekimpe, Charlotte ^{[1
]}

Roose, Elien ^{[1
]}

Tersteeg, Claudia ^{[1
]}

Joly, Berangere S. ^{[2
,3
]}

Dewaele, Aurelie ^{[1
]}

Horta, Sara ^{[1
]}

Pareyn, Inge ^{[1
]}

Vandenbulcke, Aline ^{[1
]}

Deckmyn, Hans ^{[1
]}

Feys, Hendrik B. ^{[4
,5
]}

Tellier, Edwige ^{[6
]}

Kaplanski, Gilles ^{[6
,7
]}

Scully, Marie ^{[8
,9
]}

Coppo, Paul ^{[10
,11
]}

De Meyer, Simon F. ^{[1
]}

Veyradier, Agnes ^{[2
,3
]}

Vanhoorelbeke, Karen ^{[1
]}

机构：

[1] KU Leuven Campus Kulak Kortrijk, IRF Life Sci, Lab Thrombosis Res, Etienne Sabbelaan 53, B-8500 Kortrijk, Belgium

[2] Univ Paris, Hop Lariboisiere, AP HP Nord, Serv Hematol Biol, Paris, France

[3] Univ Paris, EA3518, Inst Rech St Louis, Paris, France

[4] Belgian Red Cross Flanders, Transfus Res Ctr, Ghent, Belgium

[5] Univ Ghent, Fac Med & Hlth Sci, Ghent, Belgium

[6] Aix Marseille Univ, INSERM, INRA, C2VN, Marseille, France

[7] Aix Marseille Univ, APHM, INSERM, INRA,C2VN,Serv Med Interne, Marseille, France

[8] Univ Coll London Hosp, Dept Haematol, London, England

[9] UCLH UCL BRC, Natl Inst Hlth Res Cardiometab Programme, London, England

[10] Hop St Antoine, AP HP, Dept Hematol Clin, Paris, France

[11] Univ Paris 06, Paris, France

来源：

JOURNAL OF THROMBOSIS AND HAEMOSTASIS | 2020年 / 18卷 / 04期

关键词：

ADAMTS13; protein; autoantibodies; diagnosis; enzyme-linked immunosorbent assay; purpura thrombotic thrombocytopenic; ANTIBODY; THERAPY;

D O I：

10.1111/jth.14747

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background The biological diagnosis of immune-mediated thrombotic thrombocytopenic purpura (iTTP) is based on determination of ADAMTS13 activity (<10%) and anti-ADAMTS13 autoantibodies. ADAMTS13 antigen levels are not routinely measured in iTTP patients, but studies have shown that antigen levels are a valuable prognostic factor. Objectives To (a) report the validation of our in-house developed ADAMTS13 antigen enzyme-linked immunosorbent assay (ELISA) and determine ADAMTS13 antigen in a large cohort of healthy donor and iTTP patient plasma samples; and (b) to investigate whether ADAMTS13 antigen determination is not disturbed by the presence of anti-ADAMTS13 autoantibodies. Methods Our in-house ADAMTS13 antigen ELISA was validated in terms of sensitivity, repeatability, and reproducibility. ADAMTS13 antigen levels were determined in plasma samples from 423 healthy donors and 112 acute iTTP patients. Purified IgGs from iTTP patients were added to normal human plasma to determine whether anti-ADAMTS13 autoantibodies hampered ADAMTS13 antigen determination. Results Our in-house ADAMTS13 antigen ELISA has a detection limit of 3% and low intra-assay (coefficient of variation, %CV < 10%) and inter-assay (%CV < 18%) variability. ADAMTS13 antigen levels were significantly reduced (P < .0001) in acute iTTP patients (15 +/- 18%) compared to healthy donors (101 +/- 18%). The anti-ADAMTS13 autoantibodies in plasma of iTTP patients did not impede ADAMTS13 antigen determinations using our in-house ELISA. Conclusions Our in-house ADAMT13 antigen ELISA is a powerful tool to correctly determine ADAMTS13 antigen levels in iTTP patients, which supports routine ADAMTS13 antigen measurements in these patients to have better insight into disease prognosis.

引用

页码：985 / 990

页数：6

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