Methylene blue as a photosensitizer in the photodynamic therapy of breast cancer cell lines

被引：0

作者：

Atenco-Cuautle, J. C. ^{[1
]}

Spezzia-Mazzocco, T. ^{[2
]}

Ramos-Garcia, R. ^{[1
]}

Ramirez-San-Juan, J. C. ^{[1
]}

Ramirez-Ramirez, J. ^{[1
]}

Delgado-Lopez, M. G. ^{[3
]}

机构：

[1] Inst Nacl Astrofis Opt & Electr, Dept Opt, Luis Enrique Erro 1, Puebla 72840, Mexico

[2] Inst Nacl Astrofis Opt & Electr, CONACYT, CATEDRA, Luis Enrique Erro 1, Puebla 72840, Mexico

[3] Inst Mexicano Seguro Social, Ctr Invest Biomed Oriente, Lab Biol Celular, Km 4-5 Carretera Atlixco Metepec, Puebla 74360, Mexico

来源：

17TH INTERNATIONAL PHOTODYNAMIC ASSOCIATION WORLD CONGRESS | 2019年 / 11070卷

关键词：

photodynamic therapy; methylene blue; breast cancer; cell line; RAPID COLORIMETRIC ASSAY; APOPTOSIS; SURVIVAL; GROWTH;

D O I：

10.1117/12.2525454

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We compared MB-PDT effect on two breast cancer cell lines: MDA-MB-231 from a triple negative adenocarcinoma, and T47D from a ductal cell tumor of woman. Methylene blue concentrations at 5, 10 and 20 mu M and red light doses of 20, 40 and 60 J/cm(2) were employed. Cell viability was evaluated with the MTT test, obtaining around 80% of inhibition with 20 mu M and 60 J/cm(2) of light dose.

引用

页数：4

共 10 条

[1] Chen YJ, 2008, CANCER SCI, V99, P2019, DOI 10.1111/j.1349-7006.2008.00910.x
[2] RAPID COLORIMETRIC ASSAY FOR CELL-GROWTH AND SURVIVAL - MODIFICATIONS TO THE TETRAZOLIUM DYE PROCEDURE GIVING IMPROVED SENSITIVITY AND RELIABILITY
DENIZOT, F
LANG, R
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 89 (02) : 271 - 277
[3] Photosensitized singlet oxygen and its applications
DeRosa, MC
Crutchley, RJ
[J]. COORDINATION CHEMISTRY REVIEWS, 2002, 233 : 351 - 371
[4] Methylene blue photodynamic therapy induces selective and massive cell death in human breast cancer cells
dos Santos, Ancely F.
Terra, Leticia F.
Wailemann, Rosangela A. M.
Oliveira, Talita C.
Gomes, Vinicius de Morais
Mineiro, Marcela Franco
Meotti, Flavia Carla
Bruni-Cardoso, Alexandre
Baptista, Mauricio S.
Labriola, Leticia
[J]. BMC CANCER, 2017, 17
[5] Methylene Blue-Mediated Photodynamic Therapy Induces Mitochondria-Dependent Apoptosis in HeLa Cell
Lu, Yan
Jiao, Ruiqing
Chen, Xiaoping
Zhong, Jieying
Ji, Jianguo
Shen, Pingping
[J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2008, 105 (06) : 1451 - 1460
[6] Basic principles of photodynamic therapy
MacDonald, IJ
Dougherty, TJ
[J]. JOURNAL OF PORPHYRINS AND PHTHALOCYANINES, 2001, 5 (02) : 105 - 129
[7] RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS
MOSMANN, T
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) : 55 - 63
[8] Prasad P. N., 2004, Introduction to biophotonics
[9] Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications
Tardivo, Joao Paulo
Del Giglio, Auro
de Oliveira, Carla Santos
Gabrielli, Dino Santesso
Junqueira, Helena Couto
Tada, Dayane Batista
Severino, Divinomar
Turchiello, Rozane de Fatima
Baptista, Mauricio S.
[J]. PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2005, 2 (03) : 175 - 191
[10] Vo-Dinh Tuan., 2003, BIOMED PHOTONICS, DOI 10.1201/9780203008997-38

← 1 →