Effect of a 5-HT1A receptor agonist (8-OH-DPAT) on the external urethral sphincter activity in the rat

被引:12
作者
Chen, Shih-Ching [1 ,2 ]
Fan, Wen-Jia [3 ]
Lai, Chien-Hung [1 ,2 ]
Chen, Jia-Jin Jason [4 ]
Peng, Chih-Wei [1 ,2 ]
机构
[1] Taipei Med Univ, Dept Phys Med & Rehabil, Sch Med, Coll Med, Taipei 110, Taiwan
[2] Taipei Med Univ Hosp, Dept Phys Med & Rehabil, Taipei 110, Taiwan
[3] Natl Yang Ming Univ, Inst Physiol, Taipei 112, Taiwan
[4] Natl Cheng Kung Univ, Inst Biomed Engn, Tainan 701, Taiwan
关键词
cystometrography; electromyography; urethral flow rate; voiding; SPINAL-CORD-INJURY; URINARY-INCONTINENCE; SEROTONIN RECEPTORS; ANESTHETIZED RATS; CONSCIOUS RATS; MICTURITION; ACTIVATION; REFLEXES; CATS;
D O I
10.1016/j.jfma.2011.01.003
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background/Purpose: This study examined the effects of a 5-HT1A receptor agonist (8-OH-DPAT) on external urethral sphincter (EUS) activity in urethane-anesthetized rats. Methods: An EUS electromyogram (EMG) and intravesical pressure (IVP) were simultaneously recorded during continuous cystometrographic monitoring, to provide a quantitative evaluation of EUS activity and urethral urodynamics of voiding. Results: When examining the EUS burst activity, durations of the active (AP) and silent periods (SP) as a function of the time axis, respectively, exhibited concave- and convex-shaped curves. The burst discharges of the EUS-EMG were divided into nonvoiding and voiding burst activities based on the oscillation waves of the IVP, which were located in Phases 1 and 2 of the IVP. After 8-OH-DPAT treatment, the entire burst period in Phases 1 to 2 of the IVP was significantly prolonged. The average SP in both Phases 1 and 2 significantly increased but the average APs were not affected. Urodynamic results showed decreases in the volume threshold, contraction amplitude, and residual volume as well as an increase in the contraction duration. In addition, the amplitude of bladder high-frequency oscillatory waves in the IVP and the average urethral flow rate were reduced, but the entire voiding efficiency increased. Conclusion: The influences of 8-OH-DPAT on EUS burst activity and urodynamics were exactly detected by the sophisticated EMG analytic design, and the results could be a reference for the pharmacological treatment of patients with lower urinary tract dysfunction. Copyright (C) 2012, Elsevier Taiwan LLC & Formosan Medical Association. All rights reserved.
引用
收藏
页码:67 / 76
页数:10
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