1H-NMR metabolomics for evaluating the protective effect of Clinacanthus nutans (Burm. f) Lindau water extract against nitric oxide production in LPS-IFN- activated RAW 264.7 macrophages

被引：17

作者：

Khoo, Leng Wei ^{[1
]}

Kow, Audrey Siew Foong ^{[2
]}

Maulidiani, Maulidiani ^{[3
]}

Ang, May Yen ^{[4
]}

Chew, Won Yin ^{[4
]}

Lee, Ming Tatt ^{[5
]}

Tan, Chin Ping ^{[6
]}

Shaari, Khozirah ^{[3
]}

Tham, Chau Ling ^{[2
]}

Abas, Faridah ^{[1
,3
]}

机构：

[1] Univ Putra Malaysia, Dept Food Sci, Fac Food Sci & Technol, Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Dept Biomed Sci, Fac Med & Hlth Sci, Serdang, Selangor, Malaysia

[3] Univ Putra Malaysia, Inst Biosci, Lab Nat Prod, Serdang, Selangor, Malaysia

[4] Shimadzu Malaysia, Analyt Instrument Div, Petaling Jaya, Selangor, Malaysia

[5] UCSI Univ Kuala Lumpur Campus, Fac Pharmaceut Sci, Kuala Lumpur, Malaysia

[6] Univ Putra Malaysia, Dept Food Technol, Fac Food Sci & Technol, Serdang, Selangor, Malaysia

来源：

PHYTOCHEMICAL ANALYSIS | 2019年 / 30卷 / 01期

关键词：

anti-inflammation; Clinacanthus nutans; HPLC-MS quantification; H-1-NMR metabolomics; plant biosynthesis pathway; CHAIN FATTY-ACIDS; NF-KAPPA-B; ANTIOXIDANT ACTIVITY; INHIBITION; TRITERPENOIDS; CONSTITUENTS; BIOACTIVITY; BUTYRATE; LEAVES; CELLS;

D O I：

10.1002/pca.2789

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

IntroductionClinacanthus nutans, a small shrub that is native to Southeast Asia, is commonly used in traditional herbal medicine and as a food source. Its anti-inflammation properties is influenced by the metabolites composition, which can be determined by different binary extraction solvent ratio and extraction methods used during plant post-harvesting stage. ObjectiveEvaluate the relationship between the chemical composition of C. nutans and its anti-inflammatory properties using nuclear magnetic resonance (NMR) metabolomics approach. MethodologyThe anti-inflammatory effect of C. nutans air-dried leaves extracted using five different binary extraction solvent ratio and two extraction methods was determined based on their nitric oxide (NO) inhibition effect in lipopolysaccharide-interferon-gamma (LPS-IFN-) activated RAW 264.7 macrophages. The relationship between extract bioactivity and metabolite profiles and quantifications were established using H-1-NMR metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The possible metabolite biosynthesis pathway was constructed to further strengthen the findings. ResultsWater and sonication prepared air-dried leaves possessed the highest NO inhibition activity (IC50=190.4312.26g/mL, P<0.05). A total of 56 metabolites were tentatively identified using H-1-NMR metabolomics. A partial least square (PLS) biplot suggested that sulphur containing glucoside, sulphur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors. LC-MS/MS targeted quantification further supported sonicated water extract was among the extract that possessed the most abundant C-glycosyl flavones. ConclusionThe present study may serve as a preliminary reference for the selection of optimum extract in further C. nutans in vivo anti-inflammatory study. Clinacanthus nutans (Burm. f) Lindau is a vegetable and herb that native to Southeast Asia. Present study aims to evaluate the relationship between the chemical composition and the anti-inflammatory properties of C. nutans using NMR metabolomics approach. Water-sonication prepared air-dried leaves exhibited the highest NO inhibition activity in LPS-IFN- activated RAW 264.7 macrophages (IC50: 190.43 +/- 12.26 g/mL). Sulfur containing glucoside, sulfur containing compounds, phytosterols, triterpenoids, flavones and some organic and amino acids were among the potential NO inhibitors.

引用

页码：46 / 61

页数：16