Detection of Babesia divergens using molecular methods in anemic patients in Shandong Province, China

被引：55

作者：

Qi, Chunhua ^{[1
,2
]}

Zhou, Dong ^{[1
]}

Liu, Jianzhu ^{[1
]}

Cheng, Ziqiang ^{[1
]}

Zhang, Li ^{[1
]}

Wang, Lin ^{[1
]}

Wang, Zhenyong ^{[1
]}

Yang, Dubao ^{[1
]}

Wang, Shujing ^{[1
]}

Chai, Tongjie ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Vet Med, Res Ctr Anim Dis Control Engn Shandong Prov, Tai An 271018, Shandong, Peoples R China

[2] Cent Hosp Taian City, Tai An 271018, Shandong, Peoples R China

来源：

PARASITOLOGY RESEARCH | 2011年 / 109卷 / 01期

基金：

中国博士后科学基金;

关键词：

CATTLE; ORGANISM; DISEASE; EUROPE;

D O I：

10.1007/s00436-011-2382-8

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Babesiosis (piroplasmosis) is a zoonotic disease caused by an intraerythrocytic protozoan transmitted by Ixodes ticks. The aim of this study was to detect Babesia spp. infection using molecular methods in 377 blood samples from anemic patients. Sequence analysis showed that the 18S rRNA gene was 439 bases long by polymerase chain reaction (PCR) amplification and that the PCR products from the samples had an identical sequence (named Taian China, HM355854). BLAST search showed that the sequence was identical to the 18S rRNA sequences of Babesia divergens. The 18S rRNA sequence for Toxoplasma gondii was included as the outlier for phylogenetic analysis by using the program MEGA4.0 software. The results showed that the 18S rRNA gene sequences obtained from the present study was most closely related to B. divergens Switzerland (DQ312439) with 98.4% similarity (differing only by seven bases). The phylogenetic analysis also revealed that this sequence closely resembled B. divergens strains from other countries and belonged to the same clade. This is the first report of a human being infected by B. divergens in China.

引用

页码：241 / 245

页数：5

共 21 条

[1] Differentiation of Babesia bigemina, B-bovis, B-divergens and B-major by Western blotting -: first report of B-bovis in Austrian cattle [J].