H3K36 Trimethylation-Mediated Epigenetic Regulation is Activated by Bam and Promotes Germ Cell Differentiation During Early Oogenesis in Drosophila

被引：11

作者：

Mukai, Masanori ^{[1
,2
,3
]}

Hira, Seiji ^{[2
,4
]}

Nakamura, Katsuhiro ^{[1
,2
]}

Nakamura, Shoichi

Kimura, Hiroshi ^{[5
]}

Sato, Masanao ^{[6
]}

Kobayashi, Satoru ^{[6
,7
]}

机构：

[1] Konan Univ, Fac Sci & Engn, Dept Biol, Higashinada Ku, Kobe, Hyogo 6588501, Japan

[2] Konan Univ, Grad Sch Nat Sci, Higashinada Ku, Kobe, Hyogo 6588501, Japan

[3] Konan Univ, Inst Integrat Neurobiol, Higashinada Ku, Kobe, Hyogo 6588501, Japan

[4] Japan Soc Promot Sci, Tokyo 1020083, Japan

[5] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Sci, Yokohama, Kanagawa 2268501, Japan

[6] Natl Inst Nat Sci, Natl Inst Basic Biol, Okazaki Inst Integrat Biosci, Higashiyama Ku, Okazaki, Aichi 4448787, Japan

[7] Univ Tsukuba, Life Sci Ctr, Tsukuba Adv Res Alliance, Tsukuba, Ibaraki 3058577, Japan

来源：

BIOLOGY OPEN | 2015年 / 4卷 / 02期

关键词：

Drosophila; Germ cell; Differentiation factor; Histone modification; Transcriptional control; RNA-POLYMERASE-II; STEM-CELLS; HISTONE H3; TRANSCRIPTIONAL ELONGATION; EGG CHAMBER; METHYLATION; PROTEIN; SET2;

D O I：

10.1242/bio.201410850

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Epigenetic silencing is critical for maintaining germline stem cells in Drosophila ovaries. However, it remains unclear how the differentiation factor, Bag-of-marbles (Bam), counteracts transcriptional silencing. We found that the trimethylation of lysine 36 on histone H3 (H3K36me3), a modification that is associated with gene activation, is enhanced in Bam-expressing cells. H3K36me3 levels were reduced in flies deficient in Bam. Inactivation of the Set2 methyltransferase, which confers the H3K36me3 modification, in germline cells markedly reduced H3K36me3 and impaired differentiation. Genetic analyses revealed that Set2 acts downstream of Bam. Furthermore, orb expression, which is required for germ cell differentiation, was activated by Set2, probably through direct H3K36me3 modification of the orb locus. Our data indicate that H3K36me3-mediated epigenetic regulation is activated by bam, and that this modification facilitates germ cell differentiation, probably through transcriptional activation. This work provides a novel link between Bam and epigenetic transcriptional control.

引用

页码：119 / 124

页数：6

共 19 条

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