Strategies to evade false positives in the in situ analysis of peptide antibiotics in SDS-PAGE gels

被引：2

作者：

Anandaraj, Balaiah ^{[1
]}

Selvamanikandan, Athinarayanan ^{[1
]}

Mittal, Prabakar ^{[1
]}

Elavarasi, Natarajan ^{[1
]}

Thangadurai, Chinnathambi ^{[1
]}

Murugan, Vadivel ^{[1
]}

机构：

[1] Anna Univ, Ctr Biotechnol, Genet Engn Unit, Madras 600025, Tamil Nadu, India

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 373卷 / 02期

关键词：

D O I：

10.1016/j.ab.2007.09.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In situ activity assay is one of the promising techniques for the characterization of peptide antibiotics. This assay was carried out for the peptide purified from a new bacterial isolate Paenibacillus alvei and commercial peptide antibiotic polymixin E. Towards this, the routine and new protocols were tried. Interestingly, the unexpected result of these experiments - the "switch over activity" has led us to have further investigations. Here, we have addressed the potential problem in the methodology of in situ assay and demonstrated a foot proof protocol to evade the false positive results. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：401 / 403

页数：3

共 8 条

[1]

ANDREWS JM, 2006, J ANTIMICROB CHEMOTH, P1

[2] A MODIFIED METHOD TO DIRECTLY DETECT IN SDS-PAGE THE BACTERIOCIN OF PEDIOCOCCUS-ACIDILACTICI [J].

BHUNIA, AK ;

JOHNSON, MG .

LETTERS IN APPLIED MICROBIOLOGY, 1992, 15 (01) :5-7

[3] DIRECT DETECTION OF AN ANTIMICROBIAL PEPTIDE OF PEDIOCOCCUS-ACIDILACTICI IN SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS [J].

BHUNIA, AK ;

JOHNSON, MC ;

RAY, B .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1987, 2 (05) :319-322

[4] Contemporary assessment of antimicrobial susceptibility testing methods for polymyxin B and colistin: Review of available interpretative criteria and quality control guidelines [J].

Gales, AC ;

Reis, AO ;

Jones, RN .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (01) :183-190

[5] Tricine-SDS-PAGE [J].

Schaegger, Hermann .

NATURE PROTOCOLS, 2006, 1 (01) :16-22

[6] TRICINE SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS FOR THE SEPARATION OF PROTEINS IN THE RANGE FROM 1-KDA TO 100-KDA [J].

SCHAGGER, H ;

VONJAGOW, G .

ANALYTICAL BIOCHEMISTRY, 1987, 166 (02) :368-379

[7] Modulation of neutral protease expression in human mesangial cells by hyperglycaemic culture [J].

Wahab, NA ;

Mason, RM .

BIOCHEMICAL JOURNAL, 1996, 320 :777-783

[8] Analysis of serine proteases from marine sponges by 2-D zymography [J].

Wilkesman, Jeff G. ;

Schroeder, Heinz C. .

ELECTROPHORESIS, 2007, 28 (03) :429-436

← 1 →