Poly-amidoamine Dendrimers@Fe3O4based Electrochemiluminescent Nanomaterials for Biosensing of Liver Cancer Biomarkers

被引：9

作者：

Chikhaliwala, Priyal ^{[1
]}

Chandra, Sudeshna ^{[2
]}

机构：

[1] SVKMs NMIMS Univ, Sunandan Div Sch Sci, Dept Biol Sci, Mumbai 400056, Maharashtra, India

[2] SVKMs NMIMS Univ, Sunandan Div Sch Sci, Dept Chem, Mumbai 400056, Maharashtra, India

来源：

ELECTROANALYSIS | 2020年 / 32卷 / 11期

关键词：

Electrochemiluminescence; PAMAM dendrimers; Fe(3)O(4)nanoparticles; Alpha-fetoprotein; Glypican-3; ELECTROGENERATED CHEMILUMINESCENCE; ALPHA-FETOPROTEIN; HEPATOCELLULAR-CARCINOMA; ULTRASENSITIVE DETECTION; PAMAM DENDRIMERS; TUMOR-MARKERS; NANOPARTICLES; IMMUNOASSAY; OXIDATION; SYSTEM;

D O I：

10.1002/elan.202060075

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Electrochemiluminescence (ECL) of poly-amidoamine (PAMAM) dendrimers functionalized to Fe(3)O(4)nanoparticles (PAMAM@Fe(3)O(4)NPs) based on Ru(bpy)(3)(2+)complex is reported. Different generations of PAMAM dendrimer consisted of 5 (D5), 10 (D10), 8 (D8) and 16 (D16) surface primary amine-terminated groups. D5@Fe(3)O(4)NPs enhanced ECL emission in comparison to others. A possible mechanism is elucidated based on structural functionality of PAMAM with respect to amines, carbonyl, hydroxyl groups and size-based diffusion. ECL biosensing of alpha-fetoprotein (AFP) and glypican-3 (GPC-3) using 1 mM tri-propylamine is demonstrated using D5@Fe3O4NP@antibodies as detection probes. Detection limit for AFP and GPC-3 was 6 pg/mL and 0.03 pg/mL, respectively.

引用

页码：2404 / 2414

页数：11

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