Effects of pomegranate seed oil on oxidative stress markers, serum biochemical parameters and pathological findings in kidney and heart of streptozotocin-induced diabetic rats

被引：40
作者：
Mollazadeh, Hamid [1 ]
Sadeghnia, Hamid R. [1 ,2 ]
Hoseini, Azar [3 ]
Farzadnia, Mehdi [4 ]
Boroushaki, Mohammad Taher [1 ,3 ]
机构：
[1] Mashhad Univ Med Sci, Dept Pharmacol, Fac Med, Mashhad, Iran
[2] Mashhad Univ Med Sci, Neurocognit Res Ctr, Fac Med, Mashhad, Iran
[3] Mashhad Univ Med Sci, Pharmacol Res Ctr Med Plants, Fac Med, Mashhad, Iran
[4] Mashhad Univ Med Sci, Ghem Hosp, Canc Mol Pathol Res Ctr, Fac Med, Mashhad, Iran
来源：
RENAL FAILURE | 2016年 / 38卷 / 08期
关键词：
Diabetes mellitus; nephropathy; oxidative stress; pomegranate seed oil; streptozotocin; INDUCED NEPHROTOXICITY; PUNICIC ACID; TNF-ALPHA; INSULIN-RESISTANCE; COMPLICATIONS; INFLAMMATION; CONSUMPTION; DYSFUNCTION; INHIBITION; CREATININE;
D O I：
10.1080/0886022X.2016.1207053
中图分类号：
R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
1002 ; 100201 ;
摘要：
Purpose: Oxidative stress due to hyperglycemia is a major cause of diabetes complications. The aim of this study was to evaluate the effects of pomegranate seed oil (PSO) on serum biochemical parameters, cardiomyopathy and nephropathy induced by diabetes mellitus.Method: W/A adult rats were divided into four groups (12 each): group 1, received saline (1mL/kg), group 2, received streptozotocin (STZ, 65mg/kg, a single dose as i.p.), groups 3 and 4, received STZ+PSO (0.4 and 0.8mL/kg, daily by gavage, respectively). After three weeks, six rats of each group and one week later the remaining animals were anesthetized, blood samples were taken for measuring serum biochemical parameters. Sections of heart and kidneys were used for histopathological studies and the remaining tissues were homogenized for measuring malondialdehyde (MDA) and total sulfhydryl groups.Results: Significant elevation of serum creatinine and urea, LDL, triglyceride, glucose levels as well as urine markers, MDA levels in tissue homogenates and a significant decrease in total thiol content and serum HDL were observed in STZ-treated group as compared with control group. PSO treatment resulted in a significant decrease in tissue MDA content, serum creatinine and urea levels as well as urine markers as compared with STZ-treated group. Lipid profile was ameliorated with PSO treatment. PSO also significantly reversed STZ-induced depletion in thiol content and histological abnormality. Effect of PSO was more specific at 28th than 21th days of study.Conclusion: The results showed that PSO has a protective effect against diabetes complications in rats.
引用
收藏
页码：1256 / 1266
页数：11
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