Phosphatidylinositol-specific phospholipase c production from Bacillus thuringiensis serovar. kurstaki using potato-based media

被引：4
|
作者：
Palvannan, T [1 ]
Boopathy, R
机构：
[1] Kumaraguru Coll Technol, Dept Biotechnol, Coimbatore 641006, Tamil Nadu, India
[2] Bharathiar Univ, Dept Biotechnol, Coimbatore 641046, Tamil Nadu, India
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2005年 / 21卷 / 6-7期
关键词：
acetylcholinesterases; Bacillus thuringiensis; phosphatidylinositol-specific phospholipases C; screening; triacyl-glycerol;
D O I：
10.1007/s11274-005-0299-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Few studies on screening of the enzyme phosphatidylinositol-specific phospholipase C (PI-PLC) have been reported. Here we present a simple potato-based medium to elaborate large quantities of extracellular PI-PLC from Bacillus thuringiensis serovar. kurstaki. The enzyme activity (1.75 U/ml) was found to be maximum in the potato-extract medium supplemented with sucrose and mineral salts with a specific activity of 3.88 U/mg protein.
引用
收藏
页码：1153 / 1155
页数：3
相关论文
共 50 条
  • [1] Phosphatidylinositol-Specific Phospholipase C Production from Bacillus thuringiensis Serovar. kurstaki using Potato-Based Media
    T. Palvannan
    R. Boopathy
    World Journal of Microbiology and Biotechnology, 2005, 21 : 1153 - 1155
  • [2] Sequence of the Bacillus thuringiensis var. kurstaki phosphatidylinositol-specific phospholipase
    Daskalova, SM
    Kostadinova, S
    Ivanov, A
    BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2001, 15 (01) : 103 - 106
  • [3] Purification and some properties of phosphatidylinositol-specific phospholipase C from Bacillus thuringiensis
    Kostadinova, S
    Ivanov, A
    BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2002, 16 (02) : 121 - 126
  • [4] PURIFICATION AND PROPERTIES OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C OF BACILLUS-THURINGIENSIS
    TAGUCHI, R
    ASAHI, Y
    IKEZAWA, H
    BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 619 (01) : 48 - 57
  • [5] COMPLETE PURIFICATION OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C FROM A STRAIN OF BACILLUS-THURINGIENSIS
    IKEZAWA, H
    NAKABAYASHI, T
    SUZUKI, K
    NAKAJIMA, M
    TAGUCHI, T
    TAGUCHI, R
    JOURNAL OF BIOCHEMISTRY, 1983, 93 (06): : 1717 - 1719
  • [6] MOLECULAR CHARACTERIZATION AND SEQUENCE OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C OF BACILLUS-THURINGIENSIS
    LECHNER, M
    KUPKE, T
    STEFANOVIC, S
    GOTZ, F
    MOLECULAR MICROBIOLOGY, 1989, 3 (05) : 621 - 626
  • [7] PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C OF BACILLUS-THURINGIENSIS AS A PROBE FOR THE DISTRIBUTION OF PHOSPHATIDYLINOSITOL IN HEPATOCYTE MEMBRANES
    HIGGINS, JA
    HITCHIN, BW
    LOW, MG
    BIOCHEMICAL JOURNAL, 1989, 259 (03) : 913 - 916
  • [8] EFFECT OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C FROM BACILLUS-THURINGIENSIS ON SOLID SPINOCELLULAR CARCINOMA
    SARAFOV, D
    TRIFONOV, B
    IVANOV, A
    KAMBEROV, E
    ZAPRJANOV, Z
    PAVLOV, P
    GEORGIEVA, E
    TOXICON, 1992, 30 (02) : 187 - 195
  • [9] Modulation of Bacillus thuringiensis Phosphatidylinositol-specific Phospholipase C Activity by Mutations in the Putative Dimerization Interface
    Shi, Xiaomeng
    Shao, Chenghua
    Zhang, Xin
    Zambonelli, Carlo
    Redfield, Alfred G.
    Head, James F.
    Seaton, Barbara A.
    Roberts, Mary F.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (23) : 15607 - 15618
  • [10] Structure and function of phosphatidylinositol-specific phospholipase C from Bacillus cereus
    Liu, T
    Ryan, M
    Dahlquist, FW
    Griffith, OH
    BIOPHYSICAL JOURNAL, 1997, 72 (02) : TU383 - TU383
12345