Mouse models in burns research: Characterisation of the hypermetabolic response to burn injury

被引：34

作者：

Hew, Jonathan J. ^{[1
]}

Parungao, Roxanne J. ^{[1
]}

Shi, Huaikai ^{[1
]}

Tsai, Kevin Hung-Yueh ^{[2
]}

Kim, Sarah ^{[3
]}

Ma, Duncan ^{[1
]}

Malcolm, Josephine ^{[1
]}

Li, Zhe ^{[1
,4
]}

Maitz, Peter K. ^{[1
,4
]}

Wang, Yiwei ^{[1
]}

机构：

[1] Univ Sydney, Concord Hosp, ANZAC Res Inst, Burns Res & Reconstruct Surg, Sydney, NSW 2139, Australia

[2] Univ Sydney, Concord Hosp, ANZAC Res Inst, Adrenal Steroid Lab, Sydney, NSW 2139, Australia

[3] Univ Sydney, Concord Hosp, ANZAC Res Inst, Bone Biol Grp, Sydney, NSW 2139, Australia

[4] Concord Repatriat Gen Hosp, Burns & Reconstruct Surg Unit, Concord, NSW 2139, Australia

来源：

BURNS | 2020年 / 46卷 / 03期

关键词：

Burns injury; Hypermetabolism; Mouse model; Wound healing; Inflammation; Metabolism; ADIPOSE-TISSUE; ANIMAL-MODELS; TRAUMA;

D O I：

10.1016/j.burns.2019.09.014

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Objective: The aim of the study is to characterise burn induced hypermetabolism in a mouse model. Summary Background Data: There are many mouse models of burn injury currently available however, their use in burns research is limited by the general assumption that post-burn hypermetabolism is difficult to study in these models. Methods: Male Balb/c mice were subjected to either a small (1 cm(2)) or large (4 cm(2)) contact burn. The hypermetabolic response to burn injury was determined by measuring changes in basal energy expenditure. The hormonal and inflammatory mediators of hypermetabolism, and the catabolic alterations secondary to hypermetabolism were also examined. Results: Post-burn hypermetabolism was induced in both models of small and large burn. However, large burns resulted in prolonged wound healing, a more pronounced and sustained increase in basal energy expenditure, and a greater stress and systemic inflammatory response with profound catabolic consequences. Conclusions: In the present study, we have successfully characterised the burn induced systemic hypermetabolic response in a mouse model of small and large burn. These models may prove useful for researchers studying the complex aetiology of hypermetabolism and interventions. (C) 2019 Elsevier Ltd and ISBI. All rights reserved.

引用

页码：663 / 674

页数：12

共 30 条

[1] Animal models in burn research [J].