Protamine 1 to protamine 2 ratio correlates with dynamic aspects of DNA fragmentation in human sperm

被引:81
作者
Garcia-Peiro, Agustin [2 ]
Martinez-Heredia, Juan
Oliver-Bonet, Maria
Abad, Carlos [3 ]
Jose Amengual, Maria [4 ]
Navarro, Joaquima
Jones, Celine [5 ]
Coward, Kevin [5 ]
Gosalvez, Jaime [6 ]
Benet, Jordi [1 ]
机构
[1] Univ Autonoma Barcelona, Dept Biol Cellular Fisiol & Immunol, Unitat Biol Cellular & Genet Med, Fac Med, Bellaterra 08193, Spain
[2] Univ Autonoma Barcelona, Catedra Recerca Eugin UAB, Bellaterra 08193, Spain
[3] Serv Urol, Sabadell, Spain
[4] Consorci Hosp Parc Tauli, UDIAT, Sabadell, Spain
[5] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Obstet & Gynaecol, Oxford OX3 9DU, England
[6] Univ Autonoma Madrid, Dept Biol, Unidad Genet, Madrid, Spain
关键词
Spermatozoa; protamine; P1/P2; ratio; DNA integrity; infertility; DNA damage; CHROMATIN-STRUCTURE ASSAY; IN-VITRO FERTILIZATION; HUMAN SPERMATOZOA; MEIOTIC SEGREGATION; OXIDATIVE STRESS; PREGNANCY RATES; DISPERSION-TEST; MALE-FERTILITY; SEMEN QUALITY; PARAMETERS;
D O I
10.1016/j.fertnstert.2010.06.053
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To investigate the relationship between the protamine 1 to protamine 2 (P1/P2) ratio and the rate of sperm DNA fragmentation in sperm samples from human males with proven fertility and three different cohorts of male patients. Design: P1/P2 ratio was analyzed using acid-urea polyacrylamide acid-urea gels electrophoresis (PAGE). Sperm DNA fragmentation using sperm chromatin dispersion methodology was analyzed after 0, 4, 8, and 24 hours of incubation at 37 degrees C. Setting: University medical school and hospital. Patient(s): A total of 32 human males: six with proven fertility, seven carriers of chromosome reorganizations, nine clinical varicocele patients, and ten subclinical varicocele patients. Intervention(s): None. Main Outcome Measure(s): P1/P2 ratio, sperm DNA fragmentation (SDF) and the rate of sperm DNA fragmentation (rSDF). Result(s): P1/P2 ratio correlated with SDF and rSDF. Statistical differences were detected between fertile controls and patients for the three pathologies studied. rSDF yielded information that differed from baseline SDF. No differences were detected for P1/P2 ratio among patient groups, in reference to the three pathologies studied. Conclusion(s): SDF and rSDF correlates with P1/P2 ratio in human sperm, and statistical differences were detected when fertile controls were compared with three different cohorts of patients. (Fertil Steril(R) 2011;95:105-9. (C) 2011 by American Society for Reproductive Medicine.)
引用
收藏
页码:105 / 109
页数:5
相关论文
共 36 条
[1]   Biological and clinical significance of DNA damage in the male germ line [J].
Aitken, R. John ;
De Iuliis, Geoffry N. ;
McLachlan, Robert I. .
INTERNATIONAL JOURNAL OF ANDROLOGY, 2009, 32 (01) :46-56
[2]   Sperm protamine 1/protamine 2 ratios are related to in vitro fertilization pregnancy rates and predictive of fertilization ability [J].
Aoki, Vincent W. ;
Liu, Lihua ;
Jones, Kirtly P. ;
Hatasaka, Harry H. ;
Gibson, Mark ;
Peterson, C. Matthew ;
Carrell, Douglas T. .
FERTILITY AND STERILITY, 2006, 86 (05) :1408-1415
[3]   DNA integrity is compromised in protamine-deficient human sperm [J].
Aoki, VW ;
Moskovtsev, SI ;
Willis, J ;
Liu, LH ;
Mullen, JBM ;
Carrell, DT .
JOURNAL OF ANDROLOGY, 2005, 26 (06) :741-748
[4]   Segregation of chromosomes in sperm of reciprocal translocation carriers: a review [J].
Benet, J ;
Oliver-Bonet, M ;
Cifuentes, P ;
Templado, C ;
Navarro, J .
CYTOGENETIC AND GENOME RESEARCH, 2005, 111 (3-4) :281-290
[5]   Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of chromosomal translocation carrier patients [J].
Brugnon, F ;
Van Assche, E ;
Verheyen, G ;
Sion, B ;
Boucher, D ;
Pouly, JL ;
Janny, L ;
Devroey, P ;
Liebaers, I ;
Van Steirteghem, A .
HUMAN REPRODUCTION, 2006, 21 (03) :685-693
[6]   Sperm chromatin structure assay parameters measured after density gradient centrifugation are not predictive for the outcome of ART [J].
Bungum, Mona ;
Spano, Marcello ;
Humaidan, Peter ;
Eleuteri, Patrizia ;
Rescia, Michele ;
Giwercman, Aleksander .
HUMAN REPRODUCTION, 2008, 23 (01) :4-10
[7]   Do sperm DNA integrity tests predict pregnancy with in vitro fertilization? [J].
Collins, John A. ;
Barnhart, Kurt T. ;
Schlegel, Peter N. .
FERTILITY AND STERILITY, 2008, 89 (04) :823-831
[8]   DNA Damage in Human Spermatozoa Is Highly Correlated with the Efficiency of Chromatin Remodeling and the Formation of 8-Hydroxy-2′-Deoxyguanosine, a Marker of Oxidative Stress [J].
De Iuliis, Geoffry N. ;
Thomson, Laura K. ;
Mitchell, Lisa A. ;
Finnie, Jane M. ;
Koppers, Adam J. ;
Hedges, Andrew ;
Nixon, Brett ;
Aitken, R. John .
BIOLOGY OF REPRODUCTION, 2009, 81 (03) :517-524
[9]   Protamine 2 precursors (Pre-P2), protamine 1 to protamine 2 ratio (P1/P2), and assisted reproduction outcome [J].
de Mateo, Sara ;
Gazquez, Cristina ;
Guimera, Marta ;
Balasch, Juan ;
Meistrich, Marvin L. ;
Luis Ballesca, Jose ;
Oliva, Rafael .
FERTILITY AND STERILITY, 2009, 91 (03) :715-722
[10]   Infertile men with varicocele show a high relative proportion of sperm cells with intense nuclear damage level, evidenced by the sperm chromatin dispersion test [J].
Enciso, M ;
Muriel, L ;
Fernández, JL ;
Goyanes, V ;
Segrelles, E ;
Marcos, M ;
Montejo, JM ;
Ardoy, M ;
Pacheco, A ;
Gosálvez, J .
JOURNAL OF ANDROLOGY, 2006, 27 (01) :106-111