Epigallocatechin-3-Gallate Attenuates Unilateral Ureteral Obstruction-Induced Renal Interstitial Fibrosis in Mice

被引：27
作者：
Wang, Yanqiu [1 ]
Wang, Bowen [2 ]
Du, Feng [1 ]
Su, Xuesong [1 ]
Sun, Guangping [1 ]
Zhou, Guangyu [1 ]
Bian, Xiaohui [1 ]
Liu, Na [3 ]
机构：
[1] China Med Univ, Shengjing Hosp, Dept Nephrol, Shenyang 110004, Peoples R China
[2] China Med Univ, Shengjing Hosp, Dept Lab Med, Shenyang 110004, Peoples R China
[3] Ordos Cent Hosp, Dept Nephrol, Ordos, Peoples R China
来源：
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2015年 / 63卷 / 04期
关键词：
EGCG; UUO mice; renal fibrosis; ECM; EMT; TGF-beta/Smad; GREEN TEA; MESENCHYMAL TRANSITION; OXIDATIVE STRESS; RAT MODEL; TGF-BETA; TUBULOINTERSTITIAL FIBROSIS; PULMONARY-FIBROSIS; INFLAMMATION; EGCG; NEPHROPATHY;
D O I：
10.1369/0022155414568019
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The severity of tubulointerstitial fibrosis is regarded as an important determinant of renal prognosis. Therapeutic strategies targeting tubulointerstitial fibrosis have been considered to have potential in the treatment of chronic kidney disease. This study aims to evaluate the protective effects of (-)-epigallocatechin-3-gallate (EGCG), a green tea polyphenol, against renal interstitial fibrosis in mice. EGCG was administrated intraperitoneally for 14 days in a mouse model of unilateral ureteral obstruction (UUO). The results of our histological examination showed that EGCG alleviated glomerular and tubular injury and attenuated renal interstitial fibrosis in UUO mice. Furthermore, the inflammatory responses induced by UUO were inhibited, as represented by decreased macrophage infiltration and inflammatory cytokine production. Additionally, the expression of type I and III collagen in the kidney were reduced by EGCG, which indicated an inhibition of extracellular matrix accumulation. EGCG also caused an up-regulation in alpha-smooth muscle actin expression and a down-regulation in E-cadherin expression, indicating the inhibition of epithelial-to-mesenchymal transition. These changes were found to be in parallel with the decreased level of TGF-beta 1 and phosphorylated Smad. In conclusion, the present study demonstrates that EGCG could attenuate renal interstitial fibrosis in UUO mice, and this renoprotective effect might be associated with its effects of inflammatory responses alleviation and TGF-beta/Smad signaling pathway inhibition.
引用
收藏
页码：270 / 279
页数：10
相关论文
共 48 条
[1]   Interstitial expression of α-SMA:: an early marker of chronic renal allograft dysfunction [J].
Badid, C ;
Desmouliere, A ;
Babici, D ;
Hadj-Aissa, A ;
McGregor, B ;
Lefrancois, N ;
Touraine, JL ;
Laville, M .
NEPHROLOGY DIALYSIS TRANSPLANTATION, 2002, 17 (11) :1993-1998
[2]  
BOHLE A, 1994, EXP NEPHROL, V2, P205
[3]   TGF-β signaling in renal disease [J].
Böttinger, EP ;
Bitzer, M .
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2002, 13 (10) :2600-2610
[4]   EGCG inhibits CTGF expression via blocking NF-κB activation in cardiac fibroblast [J].
Cai, Yi ;
Yu, Shan-Shan ;
Chen, Ting-Ting ;
Gao, Si ;
Geng, Biao ;
Yu, Yang ;
Ye, Jian-Tao ;
Liu, Pei-Qing .
PHYTOMEDICINE, 2013, 20 (02) :106-113
[5]   Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines [J].
De Amicis, Francesca ;
Perri, Anna ;
Vizza, Donatella ;
Russo, Alessandra ;
Panno, Maria Luisa ;
Bonofiglio, Daniela ;
Giordano, Cinzia ;
Mauro, Loredana ;
Aquila, Saveria ;
Tramontano, Donatella ;
Ando, Sebastiano .
JOURNAL OF CELLULAR PHYSIOLOGY, 2013, 228 (10) :2054-2062
[6]   Bioactive components of tea: Cancer, inflammation and behavior [J].
de Mejia, Elvira Gonzalez ;
Vinicio Ramirez-Mares, Marco ;
Puangpraphant, Sirima .
BRAIN BEHAVIOR AND IMMUNITY, 2009, 23 (06) :721-731
[7]   Modulation of collagen type I, fibronectin and dermal fibroblast function and activity, in systemic sclerosis by the antioxidant epigallocatechin-3-gallate [J].
Dooley, Audrey ;
Xu Shi-Wen ;
Aden, Nima ;
Tranah, Thomas ;
Desai, Nirupa ;
Denton, Christopher P. ;
Abraham, David J. ;
Bruckdorfer, Richard .
RHEUMATOLOGY, 2010, 49 (11) :2024-2036
[8]   Molecular basis of renal fibrosis [J].
Eddy, AA .
PEDIATRIC NEPHROLOGY, 2000, 15 (3-4) :290-301
[9]   Cell contact-dependent epithelial-myofibroblast kinase-phospho-myosin regulation of transition via the Rho-Rho pathway [J].
Fan, Lingzhi ;
Sebe, Attila ;
Peterfi, Zalan ;
Masszi, Andras ;
Thirone, Ana C. P. ;
Rotstein, Ori D. ;
Nakano, Hiroyasu ;
McCulloch, Christopher A. ;
Szaszi, Katalin ;
Mucsi, Istvan ;
Kapus, Andras .
MOLECULAR BIOLOGY OF THE CELL, 2007, 18 (03) :1083-1097
[10]   Morphometric and Visual Evaluation of Fibrosis in Renal Biopsies [J].
Farris, Alton B. ;
Adams, Catherine D. ;
Brousaides, Nicole ;
Della Pelle, Patricia A. ;
Collins, A. Bernard ;
Moradi, Ellie ;
Smith, R. Neal ;
Grimm, Paul C. ;
Colvin, Robert B. .
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2011, 22 (01) :176-186
12345