Separation and purification of glucoamylase in aqueous two-phase systems by a two-step extraction

被引：0

作者：

Minami, NM ^{[1
]}

Kilikian, BV ^{[1
]}

机构：

[1] Univ Sao Paulo, Escola Politecn, Dept Engn Quim, BR-05424970 Sao Paulo, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 711卷 / 1-2期

关键词：

aqueous two-phase systems; glucoamylase; enzymes;

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Extraction in two steps of glucoamylase was studied in poly(ethylene glycol) (PEG) and potassium phosphate systems at pH values of 6, 7 and 9. Ten different conditions using PEG 300, 600, 1500, 4000 and 6000 were studied. The bottom phase of the first extraction step, with the enzyme, was reused in an appropriate concentration of PEG to form the second extraction step. The optimal partitioning conditions for glucoamylase separation were obtained in PEG 4000 (first step), PEG 1500 (second step) at pH 7 and resulted in a three-fold increase in glucoamylase purification. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：309 / 312

页数：4

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