Rapid Profiling of Chemical Constituents in Qingfei Paidu Granules Using High Performance Liquid Chromatography Coupled with Q Exactive Mass Spectrometry

被引:7
作者
Fu, Shuai [1 ,2 ]
Cheng, Rongrong [1 ,2 ]
Xiang, Zilei [1 ,2 ]
Deng, Zixin [1 ,2 ,3 ]
Liu, Tiangang [1 ,2 ,3 ,4 ]
机构
[1] Wuhan Univ, Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430072, Peoples R China
[2] Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430072, Peoples R China
[3] Wuhan Inst Biotechnol, Hubei Engn Lab Synthet Microbiol, Wuhan 430075, Peoples R China
[4] Chinese Acad Med Sci, Wuhan Res Ctr Infect Dis & Canc, Wuhan 430072, Peoples R China
基金
国家重点研发计划;
关键词
Chemical constituent identification; Qingfei Paidu granules; HPLC-Q Exactive Orbitrap-MS; Orbitrap traditional Chinese medicine library; RHIZOMA; IDENTIFICATION; STRATEGY;
D O I
10.1007/s10337-021-04085-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Qingfei Paidu (QFPD) granules have played a critical role during the Coronavirus Disease 2019 (COVID-19) in China. However, worldwide acceptance has been a problem because of the complex ingredients and unique theory of treatment. In this study, high-performance liquid chromatography (HPLC)-Q Exactive Orbitrap-mass spectrometry (MS) and the Orbitrap traditional Chinese medicine library (OTCML) were used to investigate the chemical constituents of QFPD granules. By comparing retention times, masses, isotope ion patterns, and MS2 profiles, 108 compounds were putatively identified using the OTCML combined with manual verification, including 12 alkaloids, 49 flavonoids, 13 terpenoids, 14 phenylpropanoids, 4 phenolic acids, 5 phenols, and 11 other phytochemicals. Of these compounds, 17 were confirmed using reference standards. In addition, representative compounds of these different chemical types were used as examples to analyze the fragmentation pathways and characteristic product ions. Moreover, 20 herbs within the QFPD granules were also identified to establish the sources of these chemical components. This is the first rapid profiling of the chemical constituents of QFPD granules using HPLC-Q Exactive Orbitrap-MS and yields valuable information for further quality control and mechanistic studies of QFPD granules.
引用
收藏
页码:1035 / 1048
页数:14
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