Evaluation of the genotoxicity of 10 selected dietary/environmental compounds with the in vitro micronucleus cytokinesis-block assay in an interlaboratory comparison

被引：25

作者：

Katic, Jelena ^{[1
]}

Cemeli, Eduardo ^{[2
]}

Baumgartner, Adolf ^{[2
]}

Laubenthal, Julian ^{[2
]}

Bassano, Irene ^{[2
]}

Stolevik, Solvor B. ^{[3
]}

Granum, Bent ^{[3
]}

Namork, Ellen ^{[3
]}

Nygaard, Unni C. ^{[3
]}

Lovik, Martinus ^{[3
]}

van Leeuwen, Danitsja ^{[4
]}

Loock, Kim Vande ^{[5
]}

Anderson, Diana ^{[2
]}

Fucic, Aleksandra ^{[1
]}

Decordier, Ilse ^{[5
]}

机构：

[1] Inst Med Res & Occupat Hlth, Zagreb 41000, Croatia

[2] Univ Bradford, Div Biomed Sci, Bradford BD7 1DP, W Yorkshire, England

[3] Norwegian Inst Publ Hlth, Oslo, Norway

[4] Maastricht Univ, Dept Hlth Risk Anal & Toxicol, Maastricht, Netherlands

[5] Vrije Univ Brussel, Lab Cellulaire Genet, Brussels, Belgium

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2010年 / 48卷 / 10期

关键词：

Micronuclei; Nuclear buds; Nucleoplasmic bridges; Dietary genotoxicants; PERIPHERAL-BLOOD LYMPHOCYTES; SISTER-CHROMATID EXCHANGES; HETEROCYCLIC AROMATIC-AMINES; N-NITROSO COMPOUNDS; BONE-MARROW CELLS; PERFUSED HUMAN PLACENTA; CHROMOSOMAL-ABERRATIONS; LIPID-PEROXIDATION; CYTOME ASSAY; HEPG2; CELLS;

D O I：

10.1016/j.fct.2010.06.030

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Complex exposure to xenobiotics is one of the reasons for the reported increase of respiratory diseases, cancer and immunological disturbances. Among such xenobiotics there are food mutagens whose effects on human health in the low level and/or chronic exposure still remains unknown. In the present manuscript, the compounds ethanol (EtOH), 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3,3',4,4'-tetrachlorobiphenyl (PCB 153), benzo[a]pyrene (BaP), 2-amino-3-methylimidazol[4,5-f]quinoline (IQ), 2-amino-1-methyl-6-phenylimidazol[4,5-b]pyridine (PhIP), N-Nitrosodimethylamine (NDMA) and acrylamide (AA) were evaluated in an interlaboratory comparison in the in vitro cytokinesis-block micronucleus assay (CBMN) with objective of assessing the induction of micronuclei, buds and nucleoplasmic bridges in dose responses. Statistically significant increase in MNBN frequency in binucleated cells was recorded by both laboratories for the compound PhIP (2.5 mu M). The compounds PCB (250 mu M) and AA (500 mu M) induced statistically significant increase of MNBN although it was recorded by one of the two laboratories. Induction of buds and nucleoplasmic bridges was only observed for BaP (100 mu M) and AA (500 mu M) by one of the laboratories. Data generated in this study may assist in the interpretation of the mother/newborn biomonitoring study being carried Out within project NewGeneris and will contribute to overall knowledge on the genotoxic potential of dietary/environmental toxicants. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：2612 / 2623

页数：12

共 130 条

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