Introgression of peanut smut resistance from landraces to elite peanut cultivars (Arachis hypogaea L.)

被引：16

作者：

Bressano, Marina ^{[1
]}

Massa, Alicia N. ^{[2
]}

Arias, Renee S. ^{[2
]}

de Blas, Francisco ^{[3
]}

Oddino, Claudio ^{[4
]}

Faustinelli, Paola C. ^{[2
]}

Soave, Sara ^{[4
]}

Soave, Juan H. ^{[4
]}

Perez, Maria A. ^{[1
]}

Sobolev, Victor S. ^{[2
]}

Lamb, Marshall C. ^{[2
]}

Balzarini, Monica ^{[1
]}

Buteler, Mario I. ^{[4
]}

Guillermo Seijo, J. ^{[5
,6
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Agr, Cordoba, Argentina

[2] USDA ARS, Natl Peanut Res Lab, Dawson, GA 31742 USA

[3] UNC, CONICET, Inst Multidisciplinario Biol Vegetal IMBIV, Cordoba, Argentina

[4] Gen Cabrera, Criadero El Carmen, Cordoba, Argentina

[5] UNNE, CONICET, Inst Bot Nordeste IBONE, Corrientes, Argentina

[6] Univ Nacl Nordeste, Fac Ciencias Exactas & Nat, Corrientes, Argentina

来源：

PLOS ONE | 2019年 / 14卷 / 02期

关键词：

LINKAGE MAP; IDENTIFICATION; DURANENSIS; IPAENSIS; CROSSES; MARKER; GENOME; GENES;

D O I：

10.1371/journal.pone.0211920

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Smut disease caused by the fungal pathogen Thecaphora frezii Carranza & Lindquist is threatening the peanut production in Argentina. Fungicides commonly used in the peanut crop have shown little or no effect controlling the disease, making it a priority to obtain peanut varieties resistant to smut. In this study, recombinant inbred lines (RILs) were developed from three crosses between three susceptible peanut elite cultivars (Arachis hypogaea L. subsp. hypogaea) and two resistant landraces (Arachis hypogaea L. subsp. fastigiata Waldron). Parents and RILs were evaluated under high inoculum pressure (12000 teliospores g(-1) of soil) over three years. Disease resistance parameters showed a broad range of variation with incidence mean values ranging from 1.0 to 35.0% and disease severity index ranging from 0.01 to 0.30. Average heritability (h(2)) estimates of 0.61 to 0.73 indicated that resistance in the RILs was heritable, with several lines (4 to 7 from each cross) showing a high degree of resistance and stability over three years. Evidence of genetic transfer between genetically distinguishable germplasm (introgression in a broad sense) was further supported by simple-sequence repeats (SSRs) and Insertion/Deletion (InDel) marker genotyping. This is the first report of smut genetic resistance identified in peanut landraces and its introgression into elite peanut cultivars.

引用

页数：18

共 53 条

[1] The study of introgressive lines of Triticum aestivum x Aegilops speltoides by in situ and SSR analyses [J].

Adonina, IG ;

Salina, EA ;

Efremova, TT ;

Pshenichnikova, TA .

PLANT BREEDING, 2004, 123 (03) :220-224

[2] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[3] Discriminating microsatellites from Macrophomina phaseolina and their potential association to biological functions [J].

Arias, R. S. ;

Ray, J. D. ;

Mengistu, A. ;

Scheffler, B. E. .

PLANT PATHOLOGY, 2011, 60 (04) :709-718

[4] New tools to screen wild peanut species for aflatoxin accumulation and genetic fingerprinting [J].

Arias, Renee S. ;

Sobolev, Victor S. ;

Massa, Alicia N. ;

Orner, Valerie A. ;

Walk, Travis E. ;

Ballard, Linda L. ;

Simpson, Sheron A. ;

Puppala, Naveen ;

Scheffler, Brian E. ;

de Blas, Francisco ;

Seijo, Guillermo J. .

BMC PLANT BIOLOGY, 2018, 18

[5]

BCCBA, AN MET 2016

[6]

BCCBA, AN MET 2014

[7]

BCCBA, AN MET 2015

[8]

BCCBA, AN MET 2017

[9] The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut [J].

Bertioli, David John ;

Cannon, Steven B. ;

Froenicke, Lutz ;

Huang, Guodong ;

Farmer, Andrew D. ;

Cannon, Ethalinda K. S. ;

Liu, Xin ;

Gao, Dongying ;

Clevenger, Josh ;

Dash, Sudhansu ;

Ren, Longhui ;

Moretzsohn, Marcio C. ;

Shirasawa, Kenta ;

Huang, Wei ;

Vidigal, Bruna ;

Abernathy, Brian ;

Chu, Ye ;

Niederhuth, Chad E. ;

Umale, Pooja ;

Araujo, Ana Claudia G. ;

Kozik, Alexander ;

Do Kim, Kyung ;

Burow, Mark D. ;

Varshney, Rajeev K. ;

Wang, Xingjun ;

Zhang, Xinyou ;

Barkley, Noelle ;

Guimaraes, Patricia M. ;

Isobe, Sachiko ;

Guo, Baozhu ;

Liao, Boshou ;

Stalker, H. Thomas ;

Schmitz, Robert J. ;

Scheffler, Brian E. ;

Leal-Bertioli, Soraya C. M. ;

Xun, Xu ;

Jackson, Scott A. ;

Michelmore, Richard ;

Ozias-Akins, Peggy .

NATURE GENETICS, 2016, 48 (04) :438-+

[10]

BOTSTEIN D, 1980, AM J HUM GENET, V32, P314

← 1 2 3 4 5 6 →