Irradiation affects cellular properties and Eph receptor expression in human melanoma cells

被引：24

作者：

Mosch, Birgit ^{[1
]}

Pietzsch, Doreen ^{[1
]}

Pietzsch, Jens ^{[1
]}

机构：

[1] Helmholtz Zentrum Dresden Rossendorf, Dept Radiopharmaceut Biol, Inst Radiopharm, Dresden, Germany

来源：

CELL ADHESION & MIGRATION | 2012年 / 6卷 / 02期

关键词：

radiation therapy; malignant skin cancer; metastasis; Eph receptors; ephrins; SRC FAMILY KINASES; TYROSINE KINASE; CANCER-CELLS; IN-VITRO; IONIZING-RADIATION; EPITHELIAL-CELLS; PROSTATE-CANCER; UP-REGULATION; TUMOR-CELLS; E-CADHERIN;

D O I：

10.4161/cam.20655

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

X-ray irradiation influences metastatic properties of tumor cells and, moreover, metastasis and cellular motility can be modified by members of the Eph receptor/ephrin family of receptor tyrosine kinases. We hypothesized that irradiation-induced changes in cellular properties relevant for metastasis in melanoma cells could be mediated by Eph receptor/ephrin signaling. In this pilot study, we analyzed one pre-metastatic (Mel-Juso) and three metastatic human melanoma (Mel-Juso-L3, A375, and A2058) cells lines and predominantly found anti-metastatic effects of X-ray irradiation with impaired cell growth, clonal growth and motility. Additionally, we observed an irradiation-induced increase in adhesion paralleled by a decrease in migration in Mel-Juso and Mel-Juso-L3 cells and, in part, also in A375 cells. We further demonstrate a decrease of EphA2 both in expression and activity at 7 d after irradiation paralleled by an upregulation of EphA3. Analyzing downstream signaling after irradiation, we detected decreased Src kinase phosphorylation, but unchanged focal adhesion kinase (FAK) phosphorylation, indicating, in part, irradiation-induced downregulation of signaling via the EphA2-Src-FAK axis in melanoma cells. However, to which extent this finding contributes to the modification of metastasis-relevant cellular properties remains to be elucidated.

引用

页码：113 / 125

页数：13

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