Removal of PCR inhibitors by silica membranes:: Evaluating the amplicor Mycobacterium tuberculosis kit

被引：43

作者：

Böddinghaus, B ^{[1
]}

Wichelhaus, TA ^{[1
]}

Brade, V ^{[1
]}

Bittner, T ^{[1
]}

机构：

[1] Univ Hosp, Inst Med Microbiol, D-60596 Frankfurt, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 10期

关键词：

D O I：

10.1128/JCM.39.10.3750-3752.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effectiveness of PCR inhibitor removal by silica membranes in combination with the Amplicor Mycobacterium tuberculosis kit was analyzed for 655 respiratory and nonrespiratory specimens. The overall inhibition rate was reduced from 12.5%, when applying the Amplicor kit alone, to 1.1% with the addition of silica membrane DNA purification.

引用

页码：3750 / 3752

页数：3

共 34 条

[1]

Abe Al-Soud W, 2000, J CLIN MICROBIOL, V38, P4463

[2]

Abu Al-Soud W, 2000, J CLIN MICROBIOL, V38, P345

[3] Purification and characterization of PCR-inhibitory components in blood cells [J].

Abu al-Soud, W ;

Rådström, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (02) :485-493

[4] Utility of PCR in diagnosing pulmonary tuberculosis [J].

Bennedsen, J ;

Thomsen, VO ;

Pfyffer, GE ;

Funke, G ;

Feldmann, K ;

Beneke, A ;

Jenkins, PA ;

Hegginbothom, M ;

Fahr, A ;

Hengstler, M ;

Cleator, G ;

Klapper, P ;

Wilkins, EGL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (06) :1407-1411

[5] Clinical evaluation of the roche AMPLICOR PCR Mycobacterium tuberculosis test for detection of M-tuberculosis in respiratory specimens [J].

Bergmann, JS ;

Woods, GL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (05) :1083-1085

[6] Rapid quantification and differentiation of human polyomavirus DNA in undiluted urine from patients after bone marrow transplantation [J].

Biel, SS ;

Held, TK ;

Landt, O ;

Niedrig, M ;

Gelderblom, HR ;

Siegert, W ;

Nitsche, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (10) :3689-3695

[7] SCREENING OF RESPIRATORY-TRACT SPECIMENS FOR THE PRESENCE OF MYCOBACTERIUM-TUBERCULOSIS BY USING THE GEN-PROBE AMPLIFIED MYCOBACTERIUM-TUBERCULOSIS DIRECT TEST [J].

BODMER, T ;

GURTNER, A ;

SCHOPFER, K ;

MATTER, L .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (06) :1483-1487

[8] Standardization and quality control of PCR analyses [J].

Burkardt, HJ .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2000, 38 (02) :87-91

[9] DIAGNOSIS OF TUBERCULOSIS BY AMPLICOR MYCOBACTERIUM-TUBERCULOSIS TEST - A MULTICENTER STUDY [J].

CARPENTIER, E ;

DROUILLARD, B ;

DAILLOUX, M ;

MOINARD, D ;

VALLEE, E ;

DUTILH, B ;

MAUGEIN, J ;

BERGOGNEBEREZIN, E ;

CARBONNELLE, B .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (12) :3106-3110

[10] Urinary inhibitors of polymerase chain reaction and ligase chain reaction and testing of multiple specimens may contribute to lower assay sensitivities for diagnosing Chlamydia trachomatis infected women [J].

Chernesky, MA ;

Jang, D ;

Sellors, J ;

Luinstra, K ;

Chong, S ;

Castriciano, S ;

Mahony, JB .

MOLECULAR AND CELLULAR PROBES, 1997, 11 (04) :243-249

← 1 2 3 4 →