Commonality among Fluoroquinolone-Resistant Sequence Type ST131 Extraintestinal Escherichia coli Isolates from Humans and Companion Animals in Australia

被引：98
作者：
Platell, Joanne L. [1 ]
Cobbold, Rowland N. [1 ]
Johnson, James R. [2 ,3 ]
Heisig, Anke [4 ]
Heisig, Peter [4 ]
Clabots, Connie [2 ,3 ]
Kuskowski, Michael A. [2 ,3 ]
Trott, Darren J. [1 ,5 ]
机构：
[1] Univ Queensland, Sch Vet Sci, Gatton, Qld, Australia
[2] Univ Minnesota, Minneapolis, MN USA
[3] VA Med Ctr, Minneapolis, MN USA
[4] Univ Hamburg, Inst Biochem & Mol Biol, Hamburg, Germany
[5] Univ Adelaide, Sch Anim & Vet Sci, Adelaide, SA, Australia
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2011年 / 55卷 / 08期
基金：
澳大利亚研究理事会;
关键词：
FIELD GEL-ELECTROPHORESIS; CTX-M-15; BETA-LACTAMASE; MULTIDRUG-RESISTANT; MOLECULAR CHARACTERISTICS; VIRULENCE GENOTYPES; STRAINS; CLONE; INFECTIONS; EMERGENCE; DOGS;
D O I：
10.1128/AAC.00306-11
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Escherichia coli sequence type 131 (ST131), an emergent multidrug-resistant extraintestinal pathogen, has spread epidemically among humans and was recently isolated from companion animals. To assess for human-companion animal commonality among ST131 isolates, 214 fluoroquinolone-resistant extraintestinal E. coli isolates ( 205 from humans, 9 from companion animals) from diagnostic laboratories in Australia, provisionally identified as ST131 by PCR, selectively underwent PCR-based O typing and bla(CTX-M-15) detection. A subset then underwent multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) analysis, extended virulence genotyping, antimicrobial susceptibility testing, and fluoroquinolone resistance genotyping. All isolates were O25b positive, except for two O16 isolates and one O157 isolate, which ( along with six O25b-positive isolates) were confirmed by MLST to be ST131. Only 12% of isolates ( 25 human, 1 canine) exhibited bla(CTX-M-15). PFGE analysis of 20 randomly selected human and all 9 companion animal isolates showed multiple instances of >= 94% profile similarity across host species; 12 isolates (6 human, 6 companion animal) represented pulsotype 968, the most prevalent ST131 pulsotype in North America (representing 23% of a large ST131 reference collection). Virulence gene and antimicrobial resistance profiles differed minimally, without host species specificity. The analyzed ST131 isolates also exhibited a conserved, host species-independent pattern of chromosomal fluoroquinolone resistance mutations. However, eight (89%) companion animal isolates, versus two (10%) human isolates, possessed the plasmid-borne qnrB gene (P < 0.001). This extensive across-species strain commonality, plus the similarities between Australian and non-Australian ST131 isolates, suggest that ST131 isolates are exchanged between humans and companion animals both within Australia and intercontinentally.
引用
收藏
页码：3782 / 3787
页数：6
相关论文
共 52 条
[41]   Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7 Salmonella, and Shigella for PulseNet [J].
Ribot, Efrain M. ;
Fair, M. A. ;
Gautom, R. ;
Cameron, D. N. ;
Hunter, S. B. ;
Swaminathan, B. ;
Barrett, Timothy J. .
FOODBORNE PATHOGENS AND DISEASE, 2006, 3 (01) :59-67
[42]   Medical and economic impact of extraintestinal infections due to Escherichia coli:: focus on an increasingly important endemic problem [J].
Russo, TA ;
Johnson, JR .
MICROBES AND INFECTION, 2003, 5 (05) :449-456
[43]   Virulence and antimicrobial resistance profiles among Escherichia coli strains isolated from human and animal wastewater [J].
Sabate, Montserrat ;
Prats, Guillem ;
Moreno, Eva ;
Balleste, Elisenda ;
Blanch, Anicet R. ;
Andreu, Antonia .
RESEARCH IN MICROBIOLOGY, 2008, 159 (04) :288-293
[44]   Escherichia coli ST131 producing CTX-M-15 in Australia [J].
Sidjabat, Hanna E. ;
Derrington, Petra ;
Nimmo, Graeme R. ;
Paterson, David L. .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2010, 65 (06) :1301-1303
[45]   INTERPRETING CHROMOSOMAL DNA RESTRICTION PATTERNS PRODUCED BY PULSED-FIELD GEL-ELECTROPHORESIS - CRITERIA FOR BACTERIAL STRAIN TYPING [J].
TENOVER, FC ;
ARBEIT, RD ;
GOERING, RV ;
MICKELSEN, PA ;
MURRAY, BE ;
PERSING, DH ;
SWAMINATHAN, B .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (09) :2233-2239
[46]   Food Reservoir for Escherichia coli Causing Urinary Tract Infections [J].
Vincent, Caroline ;
Boerlin, Patrick ;
Daignault, Danielle ;
Dozois, Charles M. ;
Dutil, Lucie ;
Galanakis, Chrissi ;
Reid-Smith, Richard J. ;
Tellier, Pierre-Paul ;
Tellis, Patricia A. ;
Ziebell, Kim ;
Manges, Amee R. .
EMERGING INFECTIOUS DISEASES, 2010, 16 (01) :88-95
[47]   Enteroaggregative Escherichia coli related to uropathogenic clonal group A [J].
Wallace-Gadsden, Faith ;
Johnson, James R. ;
Wain, John ;
Okeke, Iruka N. .
EMERGING INFECTIOUS DISEASES, 2007, 13 (05) :757-760
[48]   Comparative mutant prevention concentrations of pradofloxacin and other veterinary fluoroquinolones indicate differing potentials in preventing selection of resistance [J].
Wetzstein, HG .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (10) :4166-4173
[49]   Role of the acrAB locus in organic solvent tolerance mediated by expression of marA, soxS, or robA in Escherichia coli [J].
White, DG ;
Goldman, JD ;
Demple, B ;
Levy, SB .
JOURNAL OF BACTERIOLOGY, 1997, 179 (19) :6122-6126
[50]   Sex and virulence in Escherichia coli:: an evolutionary perspective [J].
Wirth, Thierry ;
Falush, Daniel ;
Lan, Ruiting ;
Colles, Frances ;
Mensa, Patience ;
Wieler, Lothar H. ;
Karch, Helge ;
Reeves, Peter R. ;
Maiden, Martin C. J. ;
Ochman, Howard ;
Achtman, Mark .
MOLECULAR MICROBIOLOGY, 2006, 60 (05) :1136-1151
123456