Wheat TaTIP4;1 Confers Enhanced Tolerance to Drought, Salt and Osmotic Stress in Arabidopsis and Rice

被引:14
作者
Wang, Yan [1 ]
Zhang, Yaqi [1 ]
An, Yinchao [1 ]
Wu, Jingyuan [1 ]
He, Shibin [1 ]
Sun, Lirong [1 ]
Hao, Fushun [1 ]
机构
[1] Henan Univ, Sch Life Sci, Coll Agr, State Key Lab Cotton Biol, Kaifeng 475004, Peoples R China
基金
中国国家自然科学基金;
关键词
wheat TaTIP4; 1; Arabidopsis; rice; seed germination; root growth; drought; salinity; osmotic stress; TONOPLAST AQUAPORIN; TRANSCRIPTION FACTORS; RESPONSES; GENE; PLANTS;
D O I
10.3390/ijms23042085
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tonoplast aquaporins (intrinsic proteins, TIPs) have been indicated to play important roles in plant tolerance to water deficit and salinity. However, the functions of wheat TIPs in response to the stresses are largely unknown. In this study, we observed that transgenic plants overexpressing wheat TaTIP4;1 in Arabidopsis and rice displayed clearly enhanced seed germination and seedling growth under drought, salt and osmotic stress. Compared with wild type plants, Arabidopsis and rice overexpression lines had heightened water contents, reduced leaf water loss, lowered levels of Na+, Na+/K+, H2O2 and malondialdehyde, and improved activities of catalase and/or superoxide dismutase, and increased accumulation of proline under drought, salinity and/or osmotic stresses. Moreover, the expression levels of multiple drought responsive genes clearly elevated upon water dehydration, and the transcription of some salt responsive genes was markedly induced by NaCl treatment in the overexpression lines. Also, the yeast cells containing TaTIP4;1 showed increased tolerance to NaCl and mannitol, and mutation in one of three serines of TaTIP4;1 caused decreased tolerance to the two stresses. These results suggest that TaTIP4;1 serves as an essential positive regulator of seed germination and seedling growth under drought, salt and/or osmotic stress through impacting water relations, ROS balance, the accumulation of Na+ and proline, and stimulating the expression of dozens of stress responsive genes in Arabidopsis and rice. Phosphorylation may modulate the activity of TaTIP4;1.
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页数:19
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共 37 条
[1]   The Roles of Aquaporins in Plant Stress Responses [J].
Afzal, Zunaira ;
Howton, T. C. ;
Sun, Yali ;
Mukhtar, M. Shahid .
JOURNAL OF DEVELOPMENTAL BIOLOGY, 2016, 4 (01)
[2]   The Panax ginseng PgTIP1 gene confers enhanced salt and drought tolerance to transgenic soybean plants by maintaining homeostasis of water, salt ions and ROS [J].
An, Jing ;
Cheng, Cong ;
Hu, Zhenmin ;
Chen, Haiying ;
Cai, Weiming ;
Yu, Bingjun .
ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2018, 155 :45-55
[3]   Heterologous Expression of Panax ginseng PgTIP1 Confers Enhanced Salt Tolerance of Soybean Cotyledon Hairy Roots, Composite, and Whole Plants [J].
An, Jing ;
Hu, Zhenmin ;
Che, Benning ;
Chen, Haiying ;
Yu, Bingjun ;
Cai, Weiming .
FRONTIERS IN PLANT SCIENCE, 2017, 8
[4]   Plant MYB Transcription Factors: Their Role in Drought Response Mechanisms [J].
Baldoni, Elena ;
Genga, Annamaria ;
Cominelli, Eleonora .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (07) :15811-15851
[5]   Identification of the AQP members involved in abiotic stress responses from Arabidopsis [J].
Feng, Zhi-Juan ;
Xu, Sheng-Chun ;
Liu, Na ;
Zhang, Gu-Wen ;
Hu, Qi-Zan ;
Xu, Zhao-Shi ;
Gong, Ya-Ming .
GENE, 2018, 646 :64-73
[6]   The PIP and TIP aquaporins in wheat form a large and diverse family with unique gene structures and functionally important features [J].
Forrest, Kerrie L. ;
Bhave, Mrinal .
FUNCTIONAL & INTEGRATIVE GENOMICS, 2008, 8 (02) :115-133
[7]   A novel vacuolar membrane H+-ATPase c subunit gene (ThVHAc1) from Tamarix hispida confers tolerance to several abiotic stresses in Saccharomyces cerevisiae [J].
Gao, Caiqiu ;
Wang, Yucheng ;
Jiang, Bo ;
Liu, Guifeng ;
Yu, Lili ;
Wei, Zhigang ;
Yang, Chuanping .
MOLECULAR BIOLOGY REPORTS, 2011, 38 (02) :957-963
[8]   Aquaporins Responses under Challenging Environmental Conditions and Abiotic Stress Tolerance in Plants [J].
Gautam, Arti ;
Pandey, Akhilesh Kumar .
BOTANICAL REVIEW, 2021, 87 (04) :467-495
[9]   The physiology of plant responses to drought [J].
Gupta, Aditi ;
Rico-Medina, Andres ;
Cano-Delgado, Ana I. .
SCIENCE, 2020, 368 (6488) :266-269
[10]   The complete set of genes encoding major intrinsic proteins in arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants [J].
Johanson, U ;
Karlsson, M ;
Johansson, I ;
Gustavsson, S ;
Sjövall, S ;
Fraysse, L ;
Weig, AR ;
Kjellbom, P .
PLANT PHYSIOLOGY, 2001, 126 (04) :1358-1369