Functional identification of a fatty acid Δ5 desaturase gene from Caenorhabditis elegans

被引：72
|
作者：
Michaelson, LV
Napier, JA
Lewis, M
Griffiths, G
Lazarus, CM
Stobart, AK
机构：
[1] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England
[2] Univ Bristol, Dept Agr Sci, IACR Long Ashton Res Stn, Bristol BS41 9AF, Avon, England
[3] Hort Res Int, Warwick CV35 9EF, England
来源：
FEBS LETTERS | 1998年 / 439卷 / 03期
基金：
英国生物技术与生命科学研究理事会;
关键词：
fatty acid desaturase; delta five; Caenorhabditis elegans;
D O I：
10.1016/S0014-5793(98)01385-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have identified a cDNA from the nematode worm Caenorhabditis elegans that encodes a fatty acid Delta(5) desaturase, Saccharomyces cerevisiae expressing the full-length cDNA was able to convert di-homo-gamma-linolenic acid to arachidonic acid, thus confirming Delta(5) desaturation. The 1341 hp Delta(5) desaturase sequence contained an N-terminal cytochrome b(5) domain and was located within a kilobase of the C. elegans Delta(6) desaturase on chromosome IV. With an amino acid identity of 45% it is possible that one of these genes arose from the other by gene duplication. This is the first example of a Delta(5) desaturase gene isolated from an animal. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：215 / 218
页数：4
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