Antagonistic Activity of Fungal Strains against Fusarium Crown Rot

被引:13
|
作者
Zhao, Xingli [1 ]
Hou, Dianyun [1 ]
Xu, Jiaqi [1 ]
Wang, Kaixuan [1 ]
Hu, Zhenjie [2 ]
机构
[1] Henan Univ Sci & Technol, Coll Agr, Luoyang 471000, Peoples R China
[2] Henan Univ Sci & Technol, Coll Hort & Plant Protect, Luoyang 471000, Peoples R China
来源
PLANTS-BASEL | 2022年 / 11卷 / 03期
关键词
antagonistic activity; identification; Fusarium crown rot; biocontrol gene; ANTIMICROBIAL ACTIVITY; TALAROMYCES-TRACHYSPERMUS; ANTIFUNGAL ACTIVITY; ENDOPHYTIC FUNGI; MEDICINAL-PLANTS; DIVERSITY; WHEAT; PSEUDOGRAMINEARUM; PKS; ACTINOBACTERIA;
D O I
10.3390/plants11030255
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The crown rot of wheat is a destructive soil-borne pathogen that severely reduces the yield and quality of wheat. This study aimed to screen and identify the antagonistic strains against Fusarium pseudograminearum (Fp), which is the dominant pathogen associated with the crown rot of wheat in China, and evaluate their biosynthetic potential. The antagonistic strains were screened via a dual-culture antagonism assay, and then identified by combining the morphological characteristics and internal transcribed spacer gene sequencing. The polyketide synthases (PKS-I and PKS-II) and non-ribosomal peptide synthetase (NRPS) genes in the antagonistic strains were detected via specific amplification of chromosomal DNA. Eleven out of 157 fungal strains, including six strains with matrix competition and five strains with antibiosis, were obtained. The eleven antagonistic strains belonged to the following four genera: Alternaria, Botryosphaeria, Phoma and Talaromyces. The inhibition rate of six strains with matrix competition was greater than 50%, with B. dothidea S2-22 demonstrating the highest at 80.3%. The width of the inhibition zone of T. trachyspermus R-17 among the five strains with antibiosis was the widest at 11 mm. Among the eleven antagonistic strains, three strains of A. alternata and the strain P. moricola only contained the PKS-II gene, the strain A. tenuissima contained PKS-I and PKS-II genes, three strains of B. dothidea contained PKS-II and NRPS genes, while three strains of T. trachyspermus did not contain any genes. These results demonstrated potential strains for the biocontrol of the crown rot of wheat. In particular, T. trachyspermus R-17 can be investigated further as a promising agent, and the active substances secreted by antagonistic strains may be synthesized by other pathways.
引用
收藏
页数:11
相关论文
共 50 条
  • [31] Changes in concentrations and transcripts of plant hormones in wheat seedling roots in response to Fusarium crown rot
    Gao, Yutian
    Tian, Xuejun
    Wang, Weidong
    Xu, Xiangru
    Su, Yuqing
    Yang, Jiatian
    Duan, Shuonan
    Li, Jinlong
    Xin, Mingming
    Peng, Huiru
    Sun, Qixin
    Xie, Chaojie
    Ma, Jun
    CROP JOURNAL, 2023, 11 (05): : 1441 - 1450
  • [32] Combatting Sugar Beet Root Rot: Streptomyces Strains' Efficacy against Fusarium oxysporum
    Abdelghany, Walaa R.
    Yassin, Abeer S.
    Abu-Ellail, Farrag F. B.
    Al-Khalaf, Areej A.
    Omara, Reda I.
    Hozzein, Wael N.
    PLANTS-BASEL, 2024, 13 (02):
  • [33] Potential native Trichoderma strains against Fusarium verticillioides causing post flowering stalk rot in winter maize
    Jambhulkar, Prashant P.
    Raja, M.
    Singh, Bhumica
    Katoch, Sonali
    Kumar, Shailendra
    Sharma, Pratibha
    CROP PROTECTION, 2022, 152
  • [34] Genetics of Resistance to Common Root Rot (Spot Blotch), Fusarium Crown Rot, and Sharp Eyespot in Wheat
    Su, Jun
    Zhao, Jiaojie
    Zhao, Shuqing
    Li, Mengyu
    Pang, Shuyong
    Chen, Shisheng
    Chen, Feng
    Zhen, Wenchao
    Kang, Zhensheng
    Wang, Xiaodong
    FRONTIERS IN GENETICS, 2021, 12
  • [35] Antifungal Activity of Nanochitin Whisker against Crown Rot Diseases of Wheat
    Liang, Rui
    Li, Xueyun
    Yuan, Wanling
    Jin, Songyao
    Hou, Shuaitao
    Wang, Min
    Wang, Hezhong
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2018, 66 (38) : 9907 - 9913
  • [36] Development and application of multiplex PCR for the rapid identi fi cation of four Fusarium spp. associated with Fusarium crown rot in wheat
    Deng, Siyi
    Chang, Wei
    Liu, Quanke
    Zhao, Youfu
    Liu, Jun
    Wang, Hua
    PEERJ, 2024, 12
  • [37] The effect of crop rotation and tillage practice on Fusarium crown rot and agronomic parameters of wheat in South Africa
    Theron, Johannes Stephanus
    van Coller, Gerhardus Johannes
    Rose, Lindy Joy
    Labuschagne, Johan
    Swanepoel, Pieter Andreas
    CROP PROTECTION, 2023, 166
  • [38] Genetic diversity of Australian Fusarium pseudograminearum populations causing crown rot in wheat
    Khudhair, Mohammed
    Obanor, F.
    Kazan, K.
    Gardiner, D. M.
    Aitken, E.
    McKay, A.
    Giblot-Ducray, D.
    Simpfendorfer, S.
    Thatcher, L. F.
    EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2021, 159 (04) : 741 - 753
  • [39] Isolation and Evaluation of the Antagonistic Activity of Cnidium officinale Rhizosphere Bacteria against Phytopathogenic fungi (Fusarium solani)
    Lee, Seok Hui
    Jeon, Su Hong
    Park, Jun Young
    Kim, Dae Sol
    Kim, Ji Ah
    Jeong, Hui Yeong
    Kang, Jun Won
    MICROORGANISMS, 2023, 11 (06)
  • [40] Identification of New Sources of Resistance to Crown Rot and Fusarium Head Blight in Wheat
    Shi, Shandang
    Zhao, Jingchen
    Pu, Lefan
    Sun, Daojie
    Han, Dejun
    Li, Chunlian
    Feng, Xiaojun
    Fan, Dongsheng
    Hu, Xiaoping
    PLANT DISEASE, 2020, 104 (07) : 1979 - 1985