Antagonistic Activity of Fungal Strains against Fusarium Crown Rot

被引:13
|
作者
Zhao, Xingli [1 ]
Hou, Dianyun [1 ]
Xu, Jiaqi [1 ]
Wang, Kaixuan [1 ]
Hu, Zhenjie [2 ]
机构
[1] Henan Univ Sci & Technol, Coll Agr, Luoyang 471000, Peoples R China
[2] Henan Univ Sci & Technol, Coll Hort & Plant Protect, Luoyang 471000, Peoples R China
来源
PLANTS-BASEL | 2022年 / 11卷 / 03期
关键词
antagonistic activity; identification; Fusarium crown rot; biocontrol gene; ANTIMICROBIAL ACTIVITY; TALAROMYCES-TRACHYSPERMUS; ANTIFUNGAL ACTIVITY; ENDOPHYTIC FUNGI; MEDICINAL-PLANTS; DIVERSITY; WHEAT; PSEUDOGRAMINEARUM; PKS; ACTINOBACTERIA;
D O I
10.3390/plants11030255
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The crown rot of wheat is a destructive soil-borne pathogen that severely reduces the yield and quality of wheat. This study aimed to screen and identify the antagonistic strains against Fusarium pseudograminearum (Fp), which is the dominant pathogen associated with the crown rot of wheat in China, and evaluate their biosynthetic potential. The antagonistic strains were screened via a dual-culture antagonism assay, and then identified by combining the morphological characteristics and internal transcribed spacer gene sequencing. The polyketide synthases (PKS-I and PKS-II) and non-ribosomal peptide synthetase (NRPS) genes in the antagonistic strains were detected via specific amplification of chromosomal DNA. Eleven out of 157 fungal strains, including six strains with matrix competition and five strains with antibiosis, were obtained. The eleven antagonistic strains belonged to the following four genera: Alternaria, Botryosphaeria, Phoma and Talaromyces. The inhibition rate of six strains with matrix competition was greater than 50%, with B. dothidea S2-22 demonstrating the highest at 80.3%. The width of the inhibition zone of T. trachyspermus R-17 among the five strains with antibiosis was the widest at 11 mm. Among the eleven antagonistic strains, three strains of A. alternata and the strain P. moricola only contained the PKS-II gene, the strain A. tenuissima contained PKS-I and PKS-II genes, three strains of B. dothidea contained PKS-II and NRPS genes, while three strains of T. trachyspermus did not contain any genes. These results demonstrated potential strains for the biocontrol of the crown rot of wheat. In particular, T. trachyspermus R-17 can be investigated further as a promising agent, and the active substances secreted by antagonistic strains may be synthesized by other pathways.
引用
收藏
页数:11
相关论文
共 50 条
  • [21] Fungal Metabolites with Antagonistic Activity against Fungi of Lithic Substrata
    Masi, Marco
    Petraretti, Mariagioia
    De Natale, Antonino
    Pollio, Antonino
    Evidente, Antonio
    BIOMOLECULES, 2021, 11 (02) : 1 - 13
  • [22] Interactions of Fusarium Crown Rot of Wheat with Nitrogen
    Buster, Mitchell
    Simpfendorfer, Steven
    Guppy, Christopher
    Sissons, Mike
    Flavel, Richard J.
    PLANTS-BASEL, 2023, 12 (03):
  • [23] FUSARIUM CROWN ROT OF ASPARAGUS - SOURCES OF INOCULUM
    GILBERTSON, RL
    DAMICONE, JP
    MANNING, WJ
    PHYTOPATHOLOGY, 1981, 71 (02) : 218 - 218
  • [24] Induction of systemic resistance against Fusarium crown and root rot disease by blast processing
    Elsharkawy, Mohsen Mohamed
    Hase, Tatsuya
    Yagi, Yusuke
    Shimizu, Masafumi
    Hyakumachi, Mitsuro
    JOURNAL OF PLANT INTERACTIONS, 2015, 10 (01) : 262 - 269
  • [25] INDICATIONS OF CROSS-PROTECTION AGAINST FUSARIUM CROWN AND ROOT-ROT OF TOMATO
    LOUTER, JH
    EDGINGTON, LV
    CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE, 1990, 12 (03): : 283 - 288
  • [26] On the biocontrol by Trichoderma afroharzianum against Fusarium culmorum responsible of fusarium head blight and crown rot of wheat in Algeria
    Hamza Bouanaka
    Ines Bellil
    Wahiba Harrat
    Saoussene Boussaha
    Abdelkader Benbelkacem
    Douadi Khelifi
    Egyptian Journal of Biological Pest Control, 31
  • [27] Screening of antagonistic activity of selected microorganisms against apple rot pathogens
    Grahovac, Mila S.
    Balaz, Jelica S.
    Grahovac, Jovana A.
    Dodic, Jelena M.
    Tanovic, Brankica B.
    Hrustic, Jovana G.
    Tadijan, Ivana Z.
    ROMANIAN BIOTECHNOLOGICAL LETTERS, 2014, 19 (01): : 8959 - 8965
  • [28] Fungal Pathogen Diversity and Fungicide Resistance Assessment in Fusarium Crown Rot of Wheat in the Huanghuai Region of China
    Xu, Chenghui
    Guo, Meiling
    Han, Xingmin
    Ren, Chunjiang
    Liu, Chao
    Fu, Weitao
    Qi, Junshan
    Ge, Zhiwei
    Ma, Zhonghua
    Chen, Yun
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2025, 73 (04) : 2299 - 2311
  • [29] Rhodanobacter ginsengiterrae sp nov., an antagonistic bacterium against root rot fungal pathogen Fusarium solani, isolated from ginseng rhizospheric soil
    Huo, Yue
    Kang, Jong-Pyo
    Park, Jin-Kyu
    Li, Jinfeng
    Chen, Ling
    Yang, Deok-Chun
    ARCHIVES OF MICROBIOLOGY, 2018, 200 (10) : 1457 - 1463
  • [30] Fusarium Crown and Root Rot of Tarragon in California Caused by Fusarium solani
    Koike, S. T.
    PLANT DISEASE, 2011, 95 (06) : 768 - 768