共 63 条
The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4
被引:52
作者:

Bertazzi, Daniela Trinca
论文数: 0 引用数: 0
h-index: 0
机构:
German Canc Res Ctr, DKFZ ZMBH Alliance, D-69120 Heidelberg, Germany German Canc Res Ctr, DKFZ ZMBH Alliance, D-69120 Heidelberg, Germany

Kurtulmus, Bahtiyar
论文数: 0 引用数: 0
h-index: 0
机构:
German Canc Res Ctr, DKFZ ZMBH Alliance, D-69120 Heidelberg, Germany German Canc Res Ctr, DKFZ ZMBH Alliance, D-69120 Heidelberg, Germany

Pereira, Gislene
论文数: 0 引用数: 0
h-index: 0
机构:
German Canc Res Ctr, DKFZ ZMBH Alliance, D-69120 Heidelberg, Germany German Canc Res Ctr, DKFZ ZMBH Alliance, D-69120 Heidelberg, Germany
机构:
[1] German Canc Res Ctr, DKFZ ZMBH Alliance, D-69120 Heidelberg, Germany
关键词:
STEM-CELL DIVISION;
BUDDING YEAST;
SACCHAROMYCES-CEREVISIAE;
NUCLEOTIDE EXCHANGE;
SPATIAL REGULATION;
CYCLE REGULATION;
PLASMA-MEMBRANE;
SHUTTLE VECTORS;
ELM1;
KINASE;
BUD CORTEX;
D O I:
10.1083/jcb.201101056
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
The spindle position checkpoint (SPOC) is an essential surveillance mechanism that allows mitotic exit only when the spindle is correctly oriented along the cell axis. Key SPOC components are the kinase Kin4 and the Bub2-Bfa1 GAP complex that inhibit the mitotic exit-promoting GTPase Tem1. During an unperturbed cell cycle, Kin4 associates with the mother spindle pole body (mSPB), whereas Bub2-Bfa1 is at the daughter SPB (dSPB). When the spindle is mispositioned, Bub2-Bfa1 and Kin4 bind to both SPBs, which enables Kin4 to phosphorylate Bfa1 and thereby block mitotic exit. Here, we show that the daughter cell protein Lte1 physically interacts with Kin4 and inhibits Kin4 kinase activity. Specifically, Lte1 binds to catalytically active Kin4 and promotes Kin4 hyperphosphorylation, which restricts Kin4 binding to the mSPB. This Lte1-mediated exclusion of Kin4 from the dSPB is essential for proper mitotic exit of cells with a correctly aligned spindle. Therefore, Lte1 promotes mitotic exit by inhibiting Kin4 activity at the dSPB.
引用
收藏
页码:1033 / 1048
页数:16
相关论文
共 63 条
[21]
Nud1p links astral microtubule organization and the control of exit from mitosis
[J].
Gruneberg, U
;
Campbell, K
;
Simpson, C
;
Grindlay, J
;
Schiebel, E
.
EMBO JOURNAL,
2000, 19 (23)
:6475-6488

Gruneberg, U
论文数: 0 引用数: 0
h-index: 0
机构:
Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland

Campbell, K
论文数: 0 引用数: 0
h-index: 0
机构:
Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland

Simpson, C
论文数: 0 引用数: 0
h-index: 0
机构:
Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland

Grindlay, J
论文数: 0 引用数: 0
h-index: 0
机构:
Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland

Schiebel, E
论文数: 0 引用数: 0
h-index: 0
机构:
Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland Beatson Inst Canc Res, CRC, Beatson Labs, Glasgow G61 1BD, Lanark, Scotland
[22]
Novel regulation of mitotic exit by the Cdc42 effectors Gic1 and Gic2
[J].
Höfken, T
;
Schiebel, E
.
JOURNAL OF CELL BIOLOGY,
2004, 164 (02)
:219-231

Höfken, T
论文数: 0 引用数: 0
h-index: 0
机构:
Christie Hosp NHS Trust, Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England Christie Hosp NHS Trust, Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

Schiebel, E
论文数: 0 引用数: 0
h-index: 0
机构:
Christie Hosp NHS Trust, Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England Christie Hosp NHS Trust, Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England
[23]
A role for cell polarity proteins in mitotic exit
[J].
Höfken, T
;
Schiebel, E
.
EMBO JOURNAL,
2002, 21 (18)
:4851-4862

Höfken, T
论文数: 0 引用数: 0
h-index: 0
机构:
Christie Hosp NHS Trust, Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England Christie Hosp NHS Trust, Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

Schiebel, E
论文数: 0 引用数: 0
h-index: 0
机构:
Christie Hosp NHS Trust, Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England Christie Hosp NHS Trust, Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England
[24]
Regulation of the Bub2/Bfal GAP complex by Cdc5 and cell cycle checkpoints
[J].
Hu, FH
;
Wang, YC
;
Liu, D
;
Li, YM
;
Qin, J
;
Elledge, SJ
.
CELL,
2001, 107 (05)
:655-665

Hu, FH
论文数: 0 引用数: 0
h-index: 0
机构: Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA

Wang, YC
论文数: 0 引用数: 0
h-index: 0
机构: Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA

Liu, D
论文数: 0 引用数: 0
h-index: 0
机构: Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA

Li, YM
论文数: 0 引用数: 0
h-index: 0
机构: Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA

Qin, J
论文数: 0 引用数: 0
h-index: 0
机构: Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA

Elledge, SJ
论文数: 0 引用数: 0
h-index: 0
机构:
Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA
[25]
E-Cadherin Is Required for Centrosome and Spindle Orientation in Drosophila Male Germline Stem Cells
[J].
Inaba, Mayu
;
Yuan, Hebao
;
Salzmann, Viktoria
;
Fuller, Margaret T.
;
Yamashita, Yukiko M.
.
PLOS ONE,
2010, 5 (08)

Inaba, Mayu
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA
Univ Michigan, Sch Med, Dept Cell & Dev Biol, Ann Arbor, MI USA Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA

Yuan, Hebao
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA
Univ Michigan, Sch Med, Dept Cell & Dev Biol, Ann Arbor, MI USA Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA

Salzmann, Viktoria
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA
Univ Michigan, Sch Med, Dept Cell & Dev Biol, Ann Arbor, MI USA Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA

Fuller, Margaret T.
论文数: 0 引用数: 0
h-index: 0
机构:
Stanford Univ, Dept Dev Biol, Sch Med, Stanford, CA 94305 USA
Stanford Univ, Dept Genet, Sch Med, Stanford, CA 94305 USA Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA

Yamashita, Yukiko M.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA
Univ Michigan, Sch Med, Dept Cell & Dev Biol, Ann Arbor, MI USA
Univ Michigan, Cellular & Mol Biol Program, Ann Arbor, MI 48109 USA Univ Michigan, Inst Life Sci, Ctr Stem Cell Biol, Ann Arbor, MI 48109 USA
[26]
A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes
[J].
Janke, C
;
Magiera, MM
;
Rathfelder, N
;
Taxis, C
;
Reber, S
;
Maekawa, H
;
Moreno-Borchart, A
;
Doenges, G
;
Schwob, E
;
Schiebel, E
;
Knop, M
.
YEAST,
2004, 21 (11)
:947-962

Janke, C
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Magiera, MM
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Rathfelder, N
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Taxis, C
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Reber, S
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Maekawa, H
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Moreno-Borchart, A
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Doenges, G
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Schwob, E
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Schiebel, E
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France

Knop, M
论文数: 0 引用数: 0
h-index: 0
机构: CRBM, CNRS, FRE2593, F-34293 Montpellier 5, France
[27]
Cdc14 activates Cdc15 to promote mitotic exit in budding yeast
[J].
Jaspersen, SL
;
Morgan, DO
.
CURRENT BIOLOGY,
2000, 10 (10)
:615-618

Jaspersen, SL
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA

Morgan, DO
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA Univ Calif San Francisco, Dept Physiol, San Francisco, CA 94143 USA
[28]
Spatial regulation of the guanine nucleotide exchange factor Lte1 in Saccharomyces cerevisiae
[J].
Jensen, S
;
Geymonat, M
;
Johnson, AL
;
Segal, M
;
Johnston, LH
.
JOURNAL OF CELL SCIENCE,
2002, 115 (24)
:4977-4991

Jensen, S
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Med Res, Div Yeast Genet, London NW7 1AA, England

Geymonat, M
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Med Res, Div Yeast Genet, London NW7 1AA, England

Johnson, AL
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Med Res, Div Yeast Genet, London NW7 1AA, England

Segal, M
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Med Res, Div Yeast Genet, London NW7 1AA, England

Johnston, LH
论文数: 0 引用数: 0
h-index: 0
机构: Natl Inst Med Res, Div Yeast Genet, London NW7 1AA, England
[29]
LTE1 OF SACCHAROMYCES-CEREVISIAE IS A 1435-CODON OPEN READING FRAME THAT HAS SEQUENCE SIMILARITIES TO GUANINE-NUCLEOTIDE RELEASING FACTORS
[J].
KENG, T
;
CLARK, MW
;
STORMS, RK
;
FORTIN, N
;
ZHONG, WW
;
OUELLETTE, BFF
;
BARTON, AB
;
KABACK, DB
;
BUSSEY, H
.
YEAST,
1994, 10 (07)
:953-958

KENG, T
论文数: 0 引用数: 0
h-index: 0
机构: MCGILL UNIV, DEPT BIOL, YEAST CHROMOSOME PROJECT 1, MONTREAL H3A 2B1, PQ, CANADA

CLARK, MW
论文数: 0 引用数: 0
h-index: 0
机构: MCGILL UNIV, DEPT BIOL, YEAST CHROMOSOME PROJECT 1, MONTREAL H3A 2B1, PQ, CANADA

STORMS, RK
论文数: 0 引用数: 0
h-index: 0
机构: MCGILL UNIV, DEPT BIOL, YEAST CHROMOSOME PROJECT 1, MONTREAL H3A 2B1, PQ, CANADA

FORTIN, N
论文数: 0 引用数: 0
h-index: 0
机构: MCGILL UNIV, DEPT BIOL, YEAST CHROMOSOME PROJECT 1, MONTREAL H3A 2B1, PQ, CANADA

ZHONG, WW
论文数: 0 引用数: 0
h-index: 0
机构: MCGILL UNIV, DEPT BIOL, YEAST CHROMOSOME PROJECT 1, MONTREAL H3A 2B1, PQ, CANADA

OUELLETTE, BFF
论文数: 0 引用数: 0
h-index: 0
机构: MCGILL UNIV, DEPT BIOL, YEAST CHROMOSOME PROJECT 1, MONTREAL H3A 2B1, PQ, CANADA

BARTON, AB
论文数: 0 引用数: 0
h-index: 0
机构: MCGILL UNIV, DEPT BIOL, YEAST CHROMOSOME PROJECT 1, MONTREAL H3A 2B1, PQ, CANADA

KABACK, DB
论文数: 0 引用数: 0
h-index: 0
机构: MCGILL UNIV, DEPT BIOL, YEAST CHROMOSOME PROJECT 1, MONTREAL H3A 2B1, PQ, CANADA

BUSSEY, H
论文数: 0 引用数: 0
h-index: 0
机构: MCGILL UNIV, DEPT BIOL, YEAST CHROMOSOME PROJECT 1, MONTREAL H3A 2B1, PQ, CANADA
[30]
Phosphorylation-Dependent Protein Interactions at the Spindle Midzone Mediate Cell Cycle Regulation of Spindle Elongation
[J].
Khmelinskii, Anton
;
Roostalu, Johanna
;
Roque, Helio
;
Antony, Claude
;
Schiebel, Elmar
.
DEVELOPMENTAL CELL,
2009, 17 (02)
:244-256

Khmelinskii, Anton
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Heidelberg, Zentrum Mol Biol, DKFZ ZMBH Allianz, D-69117 Heidelberg, Germany Univ Heidelberg, Zentrum Mol Biol, DKFZ ZMBH Allianz, D-69117 Heidelberg, Germany

论文数: 引用数:
h-index:
机构:

Roque, Helio
论文数: 0 引用数: 0
h-index: 0
机构:
EMBL, Cell Biol & Biophys Program, D-69117 Heidelberg, Germany Univ Heidelberg, Zentrum Mol Biol, DKFZ ZMBH Allianz, D-69117 Heidelberg, Germany

Antony, Claude
论文数: 0 引用数: 0
h-index: 0
机构:
EMBL, Cell Biol & Biophys Program, D-69117 Heidelberg, Germany Univ Heidelberg, Zentrum Mol Biol, DKFZ ZMBH Allianz, D-69117 Heidelberg, Germany

Schiebel, Elmar
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Heidelberg, Zentrum Mol Biol, DKFZ ZMBH Allianz, D-69117 Heidelberg, Germany Univ Heidelberg, Zentrum Mol Biol, DKFZ ZMBH Allianz, D-69117 Heidelberg, Germany