Immobilization of cellulase on magnetic nanoparticles for rice bran oil extraction in a magnetic fluidized bed

This paper presents a method for extracting rice bran oil using magnetic immobilized cellulase (MIC) in a magnetic fluidized bed (MFB). Cellulase was immobilized on Fe3O4/SiO x -g-P (glycydylmethacrylate) with an average grain size of 120 nm. The rice bran was hydrolyzed using MIC combined with magnetic immobilized alkaline protease to extract rice bran oil. Under intermittent conditions, the MIC concentration was 1.6 mg/g, the liquid to material ratio was 4:1, the enzymatic hydrolysis time was 150 min, and the oil yield was as high as 85.6 +/- 1.20% at 55 degrees C. The fluid in the MFB had a magnetic field strength of 0.022 T, a flow velocity of 0.005 m/s, and an oil extraction rate of 90.3%. This provides a theoretical basis for the extraction of rice bran oil using the subsequent MFB hydroenzyme method.