TRIM59 is a key regulator of growth and migration inrenal cell carcinoma

被引：13
作者：
Hu, S-H. [1 ]
Zhao, M-J. [2 ]
Wang, W-X. [3 ]
Xu, C-W. [4 ]
Wang, G-D. [5 ]
机构：
[1] Dali Univ, Affiliated Hosp, Dept Clin Lab, Dali, Peoples R China
[2] Tongji Univ, Shanghai Pulm Hosp, Sch Med, Clin Res Ctr Occupat Dis, Shanghai, Peoples R China
[3] Zhejiang Canc Hosp, Dept Chemotherapy, Hangzhou, Zhejiang, Peoples R China
[4] Fujian Med Univ Canc Hosp, Fujian Canc Hosp, Dept Pathol, Fuzhou, Fujian, Peoples R China
[5] Hebei Med Univ, Hosp 4, Dept Radiol, Shijiazhuang, Hebei, Peoples R China
来源：
CELLULAR AND MOLECULAR BIOLOGY | 2017年 / 63卷 / 05期
关键词：
TRIM59; Growth; Migration; Invasion; Renal cell carcinoma; INTERMEDIATE-FILAMENTS; SURVIVAL; PROTEINS; GENE;
D O I：
10.14715/cmb/2017.63.5.13
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Renal cell carcinoma (RCC) is the most common renal neoplasms and metastatic is common. Previous data have shown that the tripartite motif (TRIM) family proteinswere implicated in human tumoriogenesis. In this study, we aimed to investigate the role of TRIM59 in the cell growth and migration in RCC. The expression of TRIM59 in human RCC tissues was initially examined by qRT-PCR. Alentivirus-based shRNA against TRIM59 (Lv-shTRIM59) was constructed. The effects of TRIM59 knockdown on cell proliferation were examined by in vitro MTT assay, colony formation assay and in vivo a mouse xenograft model of RCC. Cell migration and invasion after knockdown of TRIM59 were also examined by transwell assay. Our data showed that the mRNA level of TRIM59 in cancerous tissues was 2-fold increased as compared with non-cancerous tissues. Knockdown of TRIM59 in a RCC cell line 786-O significantly slowed down cell proliferative rate and decreased both the colony number and sizes. In the mouse model, knockdown of TRIM59 consistently inhibited tumor growth in vivo. Moreover, it was shown that cell migration and invasion were suppressed by 68% and 50%, respectively in TRIM59-depleted 786-O cells. Our data suggest that TRIM59 may serve as a pro-oncogenic protein in promoting the progression of RCC. Knockdown of TRIM59 may be a promising strategy concerning the early detection and treatment of RCC.
引用
收藏
页码：68 / 74
页数：7
相关论文
共 25 条
[1]  
[Anonymous], COCHRANE DATABASE SY
[2]   The Bacterial Cytoskeleton [J].
Cabeen, Matthew T. ;
Jacobs-Wagner, Christine .
ANNUAL REVIEW OF GENETICS, VOL 44, 2010, 44 :365-392
[3]  
Cambiaghi V, 2012, ADV EXP MED BIOL, V770, P77
[4]   E-cadherin-integrin crosstalk in cancer invasion and metastasis [J].
Canel, Marta ;
Serrels, Alan ;
Frame, Margaret C. ;
Brunton, Valerie G. .
JOURNAL OF CELL SCIENCE, 2013, 126 (02) :393-401
[5]   Long-term Survival in Patients Undergoing Radical Nephrectomy and Inferior Vena Cava Thrombectomy: Single-Center Experience [J].
Ciancio, Gaetano ;
Manoharan, Murugesan ;
Katkoori, Devendar ;
De Los Santos, Rosely ;
Soloway, Mark S. .
EUROPEAN UROLOGY, 2010, 57 (04) :667-672
[6]   RAS and RHO GTPases in G1-phase cell-cycle regulation [J].
Coleman, ML ;
Marshall, CJ ;
Olson, MF .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2004, 5 (05) :355-366
[7]   RING Domain E3 Ubiquitin Ligases [J].
Deshaies, Raymond J. ;
Joazeiro, Claudio A. P. .
ANNUAL REVIEW OF BIOCHEMISTRY, 2009, 78 :399-434
[8]   Introducing intermediate filaments: from discovery to disease [J].
Eriksson, John E. ;
Dechat, Thomas ;
Grin, Boris ;
Helfand, Brian ;
Mendez, Melissa ;
Pallari, Hanna-Mari ;
Goldman, Robert D. .
JOURNAL OF CLINICAL INVESTIGATION, 2009, 119 (07) :1763-1771
[9]   TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity [J].
Gack, Michaela U. ;
Shin, Young C. ;
Joo, Chul-Hyun ;
Urano, Tomohiko ;
Liang, Chengyu ;
Sun, Lijun ;
Takeuchi, Osamu ;
Akira, Shizuo ;
Chen, Zhijian ;
Inoue, Satoshi ;
Jung, Jae U. .
NATURE, 2007, 446 (7138) :916-U2
[10]   The function of intermediate filaments in cell shape and cytoskeletal integrity [J].
Goldman, RD ;
Khuon, S ;
Chou, YH ;
Opal, P ;
Steinert, PM .
JOURNAL OF CELL BIOLOGY, 1996, 134 (04) :971-983
123