Flow cytometric quantification of HIV-1 Tat protein in tat-transfected Jurkat T cell lines

被引：3
|
作者：
Gibellini, D
Re, MC
Panaya, R
Venturi, E
Milani, D
La Placa, M
Zauli, G
机构：
[1] Univ Bologna, Dept Clin & Expt Med, Microbiol Sect, I-40138 Bologna, Italy
[2] Univ Ferrara, Human Anat Sect, I-44100 Ferrara, Italy
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1998年 / 221卷 / 1-2期
关键词：
HIV-1; tat protein; flow cytometry;
D O I：
10.1016/S0022-1759(98)00169-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The transactivator Tat protein represents a pivotal factor for the replication of human immunodeficiency virus type 1 (HIV-1). in this report, we describe a flow cytometry procedure designed to quantify the intracellular content of Tat protein in Jurkat CD4(+) T lymphoblastoid cell lines, stably transfected with plasmids expressing full-length Tat protein. Various expression vectors were compared for their effectiveness to yield Tat protein in Jurkat cells, and several technical parameters were analyzed to optimize the assay. This method offers a quick and efficient approach to select stably transfected cell. lines expressing different levels of specific protein. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：107 / 117
页数：11
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