Silk as substratum for cell attachment and proliferation

被引:16
作者
Chirila, Traian [1 ]
Barnard, Zeke [1 ]
Zainuddin [1 ]
Harkin, Damien [1 ]
机构
[1] Queensland Eye Inst, South Brisbane, Qld 4101, Australia
来源
PRICM 6: SIXTH PACIFIC RIM INTERNATIONAL CONFERENCE ON ADVANCED MATERIALS AND PROCESSING, PTS 1-3 | 2007年 / 561-565卷
关键词
silk fibroin; biomaterials; cell attachment; ocular surface; human limbal epithelial cells;
D O I
10.4028/www.scientific.net/MSF.561-565.1549
中图分类号
TF [冶金工业];
学科分类号
0806 ;
摘要
Silk fibroin (SF), isolated from silkworm (Bombyx mori) cocoons, is a natural biodegradable polymer. Over the past decade, there was some interest in using SF as a biomedical material. As part of a project to develop tissue-engineered constructs for the surgical restoration of the ocular surface (cornea, conjunctiva), we have investigated the capacity of SF to function as a substratum for the attachment and growth of corneal stem/progenitor cells harvested from the corneoscleral limbus of donor human corneal tissue. SF membranes were produced from cocoons following a protocol involving successive dissolution steps, filtration, dialysis, evaporation, and methanol treatment. Human limbal epithelial cells were harvested from donor tissue and seeded onto SF membranes. After 5 days, the culture was fixed and stained with specific agents to visualize the cells. The study indicated profuse cellular attachment and growth. SF membranes appear to be suitable as a substratum for the repair of damaged ocular surface.
引用
收藏
页码:1549 / 1552
页数:4
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