Structural characterization and immune enhancement activity of a novel polysaccharide from Moringa oleifera leaves

被引：119
作者：
Li, Chao [1 ,3 ]
Dong, Zhuping [1 ]
Zhang, Bin [1 ,3 ]
Huang, Qiang [1 ,3 ]
Liu, Guang [2 ]
Fu, Xiong [1 ,3 ]
机构：
[1] South China Univ Technol, Natl Joint Res Ctr Trop Hlth Foods, Sch Food Sci & Engn, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou 510640, Peoples R China
[2] Guangdong Acad Agr Sci, Minist Agr & Rural Affairs, Guangdong Key Lab Agr Prod Proc, Key Lab Funct Foods,Sericultural & Agrifood Res I, Guangzhou, Peoples R China
[3] Overseas Expertise Intro Ctr Discipline Innovat F, Ctr 111, Guangzhou, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2020年 / 234卷 / 234期
基金：
中国国家自然科学基金;
关键词：
Moringa oleifera; Polysaccharides; Linkage analysis; Immunoactivity; Macrophages; MACROPHAGE IMMUNOMODULATORY ACTIVITY; STRUCTURE IDENTIFICATION; ARABINOGALACTAN; ANTIOXIDANT;
D O I：
10.1016/j.carbpol.2020.115897
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A novel polysaccharide, designated as MOP-3, was extracted and isolated from the leaves of Moringa oleifera. Structural characterization showed that MOP-3 had a molecular weight (MW) of 4.033 x 10(6) Da and was composed of arabinose, glucose and galactose with a molar ratio of 47.73:1.00:57.65. Methylation and nuclear magnetic resonance (NMR) analysis revealed that the main glycosidic linkage types of MOP-3 were (1 -> 3,6)-beta-D-Galp, (1 -> 6)-beta-D-Galp, (1 -> 5)-alpha-L-Araf, and T-alpha-L-Araf. MOP-3 containing no lipopolysaccharides showed no direct cytotoxicity to the macrophage RAW 264.7 cells within the tested concentrations. The immunomodulatory assay suggested that MOP-3 could significantly enhance pinocytic capacity and increase the secretion of reactive oxygen species (ROS), nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) by up-regulating the corresponding mRNA expression levels in RAW 264.7 cells. Our findings demonstrated that MOP-3 could potentially be used as a functional food for the hypoimmunity therapies.
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页数：8
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