Different activities of the largest subunit of replication protein A cooperate during SV40 DNA replication

被引：5
作者：
Taneja, Poonam
Boche, Irene
Hartmann, Hella
Nasheuer, Heinz-Peter
Grosse, Frank
Fanning, Ellen
Weisshart, Klaus
机构：
[1] Leibniz Inst Age Res Fritz Lipmann Inst, D-07745 Jena, Germany
[2] Inst Mol Biotechnol, D-07745 Jena, Germany
[3] Natl Univ Ireland Univ Coll Galway, Dept Biochem, Galway, Ireland
来源：
FEBS LETTERS | 2007年 / 581卷 / 21期
关键词：
SV40 DNA replication; replication protein a; protein-protein interaction;
D O I：
10.1016/j.febslet.2007.07.038
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Replication protein A (RPA) is a stable heterotrimeric complex consisting of p70, p32 and p14 subunits. The protein plays a crucial role in SV40 minichromosome replication. Peptides of p70 representing interaction sites for the smaller two subunits, DNA as well as the viral initiator protein large T-antigen (Tag) and the cellular DNA polymerase a-primase (Pol) all interfered with the replication process indicating the importance of the different p70 activities in this process. Inhibition by the peptide disrupting protein-protein interactions was observed only during the pre-initiation stage prior to primer synthesis, suggesting the formation of a stable initiation complex between RPA, Tag and Pol at the primer end. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：3973 / 3978
页数：6
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