Polyoxometalates as peroxidase mimetics and their applications in H2O2 and glucose detection

被引：112

作者：

Wang, Jingjing ^{[1
]}

Han, Dongxue ^{[2
]}

Wang, Xiaohong ^{[1
]}

Qi, Bin ^{[1
]}

Zhao, Meisheng ^{[3
]}

机构：

[1] NE Normal Univ, Key Lab Polyoxometalate Sci, Minist Educ, Fac Chem, Changchun 130024, Peoples R China

[2] Chinese Acad Sci, Changchun Inst Appl Chem, Beijing 100864, Peoples R China

[3] Jilin Univ, Clin Hosp 2, Hosp Eye, Changchun 130020, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2012年 / 36卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Polyoxometalates; Peroxidase-like; Colorimetric detection; H2O2; Glucose; HYDROGEN-PEROXIDE; COMPOSITE FILM; NANOPARTICLES; NANOTUBES; SENSOR; INHIBITORS; ENZYME; BLUE; DNA;

D O I：

10.1016/j.bios.2012.03.031

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Polyoxometalates (H3PW12O40, H4SiW12O40 and H3PMo12O40) have been proven to possess intrinsic peroxidase-like activity for the first time, which can catalyze oxidation of the peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to form a blue color in aqueous solution. Among them, H3PW12O40 (PW12) exhibits higher catalytic activity to TMB than natural enzyme HRP and other two POMs. In addition, H3PW12O40/graphene exhibited higher activity than H3PW12O40 in this catalytic oxidation reaction due to the effect of graphene in promoting the electron transfer between the substrate and catalyst. POMs/H2O2/TMB system provides a simple, accurate approach to colorimetric detection for H2O2 or glucose. The colorimetric method based on POMs showed good response toward H2O2 and glucose detection with a linear range from 1.34 x 10(-7) to 6.7 x 10(-5) mol/L and 1 x 10(-7) to 1 x 10(-4) mol/L, respectively. The results showed that it is a simple, cheap, more convenient, highly selective, sensitive, and easy handling colorimetric assay. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：18 / 21

页数：4

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