Comprehensive Proteomic Characterization of Ontogenic Changes in Hematopoietic Stem and Progenitor Cells

被引：30

作者：

Jassinskaja, Maria ^{[1
]}

Johansson, Emil ^{[1
]}

Kristiansen, Trine Ahn ^{[1
]}

Akerstrand, Hugo ^{[1
]}

Sjoholm, Kristoffer ^{[2
]}

Hauri, Simon ^{[2
]}

Malmstrom, Johan ^{[2
]}

Yuan, Joan ^{[1
]}

Hansson, Jenny ^{[1
]}

机构：

[1] Lund Univ, Lund Stem Cell Ctr, Div Mol Hematol, S-22184 Lund, Sweden

[2] Lund Univ, Dept Clin Sci, Div Infect Med, S-22184 Lund, Sweden

来源：

CELL REPORTS | 2017年 / 21卷 / 11期

基金：

瑞典研究理事会;

关键词：

OXIDATIVE-STRESS; SELF-RENEWAL; FETAL LIVER; EMERGENCY GRANULOPOIESIS; MULTIPOTENT PROGENITORS; CHILDHOOD LEUKEMIA; HSCS; DIFFERENTIATION; IDENTIFICATION; REGULATORS;

D O I：

10.1016/j.celrep.2017.11.070

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Hematopoietic stem and progenitor cells (HSPCs) in the fetus and adult possess distinct molecular landscapes that regulate cell fate and change their susceptibility to initiation and progression of hematopoietic malignancies. Here, we applied in-depth quantitative proteomics to comprehensively describe and compare the proteome of fetal and adult HSPCs. Our data uncover a striking difference in complexity of the cellular proteomes, with more diverse adult-specific HSPC proteomic signatures. The differential protein content in fetal and adult HSPCs indicate distinct metabolic profiles and protein complex stoichiometries. Additionally, adult characteristics include an arsenal of proteins linked to viral and bacterial defense, as well as protection against ROS-induced protein oxidation. Further analyses show that interferon a, as well as Neutrophil elastase, has distinct functional effects in fetal and adult HSPCs. This study provides a rich resource aimed toward an enhanced mechanistic understanding of normal and malignant hematopoiesis during fetal and adult life.

引用

页码：3285 / 3297

页数：13

共 71 条

[1] In Utero Exposure to Benzene Disrupts Fetal Hematopoietic Progenitor Cell Growth via Reactive Oxygen Species [J].

Badham, Helen J. ;

Winn, Louise M. .

TOXICOLOGICAL SCIENCES, 2010, 113 (01) :207-215

[2] Autoreactivity by design: Innate B and T lymphocytes [J].

Bendelac, A ;

Bonneville, M ;

Kearney, JF .

NATURE REVIEWS IMMUNOLOGY, 2001, 1 (03) :177-186

[3] Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics [J].

Boersema, Paul J. ;

Raijmakers, Reinout ;

Lemeer, Simone ;

Mohammed, Shabaz ;

Heck, Albert J. R. .

NATURE PROTOCOLS, 2009, 4 (04) :484-494

[4] Identification of a new intrinsically timed developmental checkpoint that reprograms key hematopoietic stem cell properties [J].

Bowie, Michelle B. ;

Kent, David G. ;

Dykstra, Brad ;

McKnight, Kristen D. ;

McCaffrey, Lindsay ;

Hoodless, Pamela A. ;

Eaves, Connie J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (14) :5878-5882

[5] Hematopoietic stem cells proliferate until after birth and show a reversible phase-specific engraftment defect [J].

Bowie, Michelle B. ;

McKnight, Kristen D. ;

Kent, David G. ;

McCaffrey, Lindsay ;

Hoodless, Pamela A. ;

Eaves, Connie J. .

JOURNAL OF CLINICAL INVESTIGATION, 2006, 116 (10) :2808-2816

[6] Acute childhood leukaemia and residence next to petrol stations and automotive repair garages: the ESCALE study (SFCE) [J].

Brosselin, P. ;

Rudant, J. ;

Orsi, L. ;

Leverger, G. ;

Baruchel, A. ;

Bertrand, Y. ;

Nelken, B. ;

Robert, A. ;

Michel, G. ;

Margueritte, G. ;

Perel, Y. ;

Mechinaud, F. ;

Bordigoni, P. ;

Hemon, D. ;

Clavel, J. .

OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, 2009, 66 (09) :598-606

[7] Identification of Regulatory Networks in HSCs and Their Immediate Progeny via Integrated Proteome, Transcriptome, and DNA Methylome Analysis [J].

Cabezas-Wallscheid, Nina ;

Klimmeck, Daniel ;

Hansson, Jenny ;

Lipka, Daniel B. ;

Reyes, Alejandro ;

Wang, Qi ;

Weichenhan, Dieter ;

Lier, Amelie ;

von Paleske, Lisa ;

Renders, Simon ;

Wuensche, Peer ;

Zeisberger, Petra ;

Brocks, David ;

Gu, Lei ;

Herrmann, Carl ;

Haas, Simon ;

Essers, Marieke A. G. ;

Brors, Benedikt ;

Eils, Roland ;

Huber, Wolfgang ;

Milsom, Michael D. ;

Plass, Christoph ;

Krijgsveld, Jeroen ;

Trumpp, Andreas .

CELL STEM CELL, 2014, 15 (04) :507-522

[8] The Lin28b-let-7-Hmga2 axis determines the higher self-renewal potential of fetal haematopoietic stem cells [J].

Copley, Michael R. ;

Babovic, Sonja ;

Benz, Claudia ;

Knapp, David J. H. F. ;

Beer, Philip A. ;

Kent, David G. ;

Wohrer, Stefan ;

Treloar, David Q. ;

Day, Christopher ;

Rowe, Keegan ;

Mader, Heidi ;

Kuchenbauer, Florian ;

Humphries, R. Keith ;

Eaves, Connie J. .

NATURE CELL BIOLOGY, 2013, 15 (08) :916-U341

[9] Origin of blood cells and HSC production in the embryo [J].

Costa, Guilherme ;

Kouskoff, Valerie ;

Lacaud, Georges .

TRENDS IN IMMUNOLOGY, 2012, 33 (05) :215-223

[10] MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification [J].

Cox, Juergen ;

Mann, Matthias .

NATURE BIOTECHNOLOGY, 2008, 26 (12) :1367-1372

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