Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration

被引：10
|
作者：
Azmi, Siti Maisura [1 ]
Salih, Mohamed [1 ,2 ]
Abdelrazeg, Samar [1 ,2 ]
Roslan, Fatin Fazrina [1 ]
Mohamed, Rafeezul [1 ]
Jie, Tan Jun [1 ]
Shaharuddin, Bakiah [1 ]
机构：
[1] Univ Sains Malaysia, Adv Med & Dent Inst, Regenerat Med Cluster, SAINS BERTAM, Kepala Batas 13200, Penang, Malaysia
[2] Natl Univ Sudan, Fac Med Lab Sci, Khartoum, Sudan
来源：
REGENERATIVE MEDICINE | 2020年 / 15卷 / 03期
关键词：
corneal characterization; corneal epithelial cells; ocular surface regeneration; umbilical cord derived-mesenchymal stem cells; DIFFERENTIATION; P63; SENESCENCE; MODEL;
D O I：
10.2217/rme-2019-0103
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Aim: As a strategy to improve the outcome of ex vivo cultivated corneal epithelial transplantation, the role of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) is investigated in promoting corneal epithelial growth and functions. Materials & methods: Human telomerase-immortalized corneal epithelial cells were characterized and its functions evaluated by scratch migration assay, cellular senescence, HLA expression and spheres formation with hUC-MSC. Results: Expression of corneal epithelial markers was influenced by the duration and method of co-culture. Indirect co-culture improved cellular migration and delayed senescence when treated after 3 and 5 days. hUC-MSC downregulated expression of HLA Class I and II in IFN-gamma -stimulated human telomerase-immortalized corneal epithelial cells. Conclusion: hUC-MSC promote corneal epithelial growth and functions after treatment with hUC-MSC.
引用
收藏
页码：1381 / 1397
页数：17
相关论文
共 50 条
  • [1] Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal epithelial regeneration (vol 15, pg 1381, 2020)
    Azmi, Siti Maisura
    Salih, Mohamed
    Abdelrazeg, Samar
    Roslan, Fatin Fazrina
    Mohamed, Rafeezul
    Tan, Jun Jie
    Shaharuddin, Bakiah
    REGENERATIVE MEDICINE, 2020, 15 (07) : 1935 - 1935
  • [2] Human Umbilical Cord-Mesenchymal Stem Cells Promote Extracellular Matrix Remodeling in Microglia
    Lombardo, Marta Tiffany
    Gabrielli, Martina
    Julien-Marsollier, Florence
    Faivre, Valerie
    Le Charpentier, Tifenn
    Bokobza, Cindy
    D'Aliberti, Deborah
    Pelizzi, Nicola
    Halimi, Camilla
    Spinelli, Silvia
    Van Steenwinckel, Juliette
    Verderio, Elisabetta A. M.
    Gressens, Pierre
    Piazza, Rocco
    Verderio, Claudia
    CELLS, 2024, 13 (19)
  • [3] Human Umbilical Cord-Derived Mesenchymal Stem Cells Promote Corneal Epithelial Repair In Vitro
    Kacham, Santhosh
    Bhure, Tejal Sunil
    Eswaramoorthy, Sindhuja D.
    Naik, Gaurav
    Rath, Subha Narayan
    Parcha, Sreenivasa Rao
    Basu, Sayan
    Sangwan, Virender Singh
    Shukla, Sachin
    CELLS, 2021, 10 (05)
  • [4] Metformin potentiates immunosuppressant activity and adipogenic differentiation of human umbilical cord-mesenchymal stem cells
    Bajetto, Adriana
    Pattarozzi, Alessandra
    Sirito, Rodolfo
    Barbieri, Federica
    Florio, Tullio
    INTERNATIONAL IMMUNOPHARMACOLOGY, 2023, 124
  • [5] EFFICACY AND SAFETY OF UMBILICAL CORD-MESENCHYMAL STEM CELLS TRANSPLANTATION FOR TREATING OSTEOARTHRITIS
    Liu, C.
    Yang, Y.
    He, G.
    OSTEOARTHRITIS AND CARTILAGE, 2017, 25 : S389 - S389
  • [6] In vitro potential of human mesenchymal stem cells for corneal epithelial regeneration
    Nieto-Nicolau, Nuria
    Martin-Antonio, Beatriz
    Muller-Sanchez, Claudia
    Casaroli-Marano, Ricardo P.
    REGENERATIVE MEDICINE, 2020, 15 (03) : 1409 - 1426
  • [7] Collagen scaffold combined with human umbilical cord-mesenchymal stem cells transplantation for acute complete spinal cord injury
    Deng, Wu-Sheng
    Ma, Ke
    Liang, Bing
    Liu, Xiao-Yin
    Xu, Hui-You
    Zhang, Jian
    Shi, Heng-Yuan
    Sun, Hong-Tao
    Chen, Xu-Yi
    Zhang, Sai
    NEURAL REGENERATION RESEARCH, 2020, 15 (09) : 1686 - 1700
  • [8] Effectiveness and safety of human umbilical cord-mesenchymal stem cells for treating type 2 diabetes mellitus
    Lian, Xiao-Fen
    Lu, Dong-Hui
    Liu, Hong-Li
    Liu, Yan-Jing
    Han, Xiu-Qun
    Yang, Yang
    Lin, Yuan
    Zeng, Qing-Xiang
    Huang, Zheng-Jie
    Xie, Feng
    Huang, Cai-Hao
    Wu, Hong-Mei
    Long, Ai-Mei
    Deng, Ling-Ping
    Zhang, Fan
    WORLD JOURNAL OF DIABETES, 2022, 13 (10) : 877 - 887
  • [9] Human umbilical cord derived mesenchymal stem cells in peripheral nerve regeneration
    Bojanic, Christine
    To, Kendrick
    Zhang, Bridget
    Mak, Christopher
    Khan, Wasim S.
    WORLD JOURNAL OF STEM CELLS, 2020, 12 (04): : 288 - 302
  • [10] Collagen scaffold combined with human umbilical cord-mesenchymal stem cells transplantation for acute complete spinal cord injury
    Wu-Sheng Deng
    Ke Ma
    Bing Liang
    Xiao-Yin Liu
    Hui-You Xu
    Jian Zhang
    Heng-Yuan Shi
    Hong-Tao Sun
    Xu-Yi Chen
    Sai Zhang
    Neural Regeneration Research, 2020, 15 (09) : 1686 - 1700
12345