Transglutaminase-catalyzed preparation of human elastin-like polypeptide-based three-dimensional matrices for cell encapsulation

被引：26

作者：

Bandiera, Antonella ^{[1
]}

机构：

[1] Univ Trieste, Dipartimento Sci Vita, I-34127 Trieste, Italy

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2011年 / 49卷 / 04期

关键词：

Elastin; Encapsulation; Enzymatic cross-linking; Matrix; EXPRESSED HUMAN ELASTIN; HUMAN TROPOELASTIN; HYDROGELS; BIOMATERIALS; CULTURE; MIMICS;

D O I：

10.1016/j.enzmictec.2011.06.012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Elastin is a specialized component of the extracellular matrix and constitutes an interesting model for bio-inspired material design with a high potential for applications in biomedical and biotechnological fields. In this work, we have focused on the set up of a simple, enzymatic method for the preparation of three-dimensional matrices based on an human elastin-like biomimetic polypeptide. The matrices were described and assayed as culture substrate for cell seeding and growth. An advantage of the here-presented method is the use of mild conditions that preserve cell viability. This can be a powerful tool for many applications that require direct embedding of cells in the matrix. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：347 / 352

页数：6

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