Rhodiola sachalinesis induces the expression of inducible nitric oxide synthase gene by murine fetal hepatocytes (BNL CL.2)

被引：5

作者：

Pae, HO

Seo, WG

Oh, GS

Kim, NY

Kim, YM

Kwon, TO

Shin, MK

Chai, KY

Chung, HT ^{[1
]}

机构：

[1] Wonkwang Univ, Sch Med, Dept Microbiol & Immunol, Chonbuk 570749, South Korea

[2] Wonkwang Univ, MRRC, Chonbuk 570749, South Korea

来源：

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY | 2001年 / 23卷 / 01期

关键词：

Rhodiola sachalinensis; nitric oxide; hepatocytes; inducible nitric oxide synthase;

D O I：

10.1081/IPH-100102564

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have examined the effect of the aqueous extract of Rhodiola sachalinensis root (RSE), a traditional herbal medicine, on nitric oxide (NO) synthesis in murine fetal hepatocytes (BNL CL.2) by measuring the stable end-product nitrite and the mRNA of inducible NO synthase (iNOS). Interferon-gamma (IFN-gamma) by itself failed to induce NO synthesis in BNL CL.2 cells. RSE also did not elicit NO synthesis at concentrations up to 1000 mug/ml, but dose- and time-dependently induced NO synthesis in the presence of IFN-gamma in BNL CL.2 cells. Whereas RSE or IFN-gamma failed to induce detectable levels of iNOS mRNA, a combination of RSE and IFN-gamma, markedly induced iNOS mRNA in BNL CL.2 cells. Thus, we found that RSE triggered IFN-gamma -primed BNL CL.2 cells to synthesize NO by inducing iNOS gene expression. The capability of RSE to induce NO synthesis might be related to the therapeutic efficacy of RSE on the liver diseases.

引用

页码：25 / 33

页数：9

共 16 条

[1] β-amyloid stimulation of inducible nitric-oxide synthase in astrocytes is interleukin-1β- and tumor necrosis factor-α (TNFα)-dependent, and involves a TNFα receptor-associated factor- and NFκB-inducing kinase-dependent signaling mechanism [J].