A serial dilution microfluidic device using a ladder network generating logarithmic or linear concentrations

被引:98
作者
Kim, Choong [1 ,2 ]
Lee, Kangsun [1 ]
Kim, Jong Hyun [1 ]
Shin, Kyeong Sik [1 ]
Lee, Kyu-Jung [2 ]
Kim, Tae Song [1 ]
Kang, Ji Yoon [1 ]
机构
[1] Korea Inst Sci & Technol, Nanobiores Ctr, Seoul 136791, South Korea
[2] Korea Univ, Dept Mech Engn, Seoul, South Korea
关键词
D O I
10.1039/b714536e
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this paper, we propose a serial dilution microfluidic chip which is able to generate logarithmic or linear step-wise concentrations. These concentrations were generated via adjustments in the flow rate of two converging fluids at the channel junctions of the ladder network. The desired dilution ratios are almost independent of the flow rate or diffusion length of molecules, as the dilution device is influenced only by the ratio of volumetric flow rates. Given a set of necessary dilution ratios, whether linear or logarithmic, a serial dilution chip can be constructed via the modification of a microfluidic resistance network. The design principle was suggested and both the logarithmic and linear dilution chips were fabricated in order to verify their performance in accordance with the fluorescence intensity. The diluted concentrations of a fluorescein solution in the microfluidic device evidenced relatively high linearity, and the cytotoxicity test of MCF-7 breast cancer cells via the logarithmic dilution chip was generally consistent with the results generated with manual dilution.
引用
收藏
页码:473 / 479
页数:7
相关论文
共 18 条
  • [1] Serial dilution microchip for cytotoxicity test
    Bang, H
    Lim, SH
    Lee, YK
    Chung, S
    Chung, C
    Han, DC
    Chang, JK
    [J]. JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2004, 14 (08) : 1165 - 1170
  • [2] Microstructure for efficient continuous flow mixing
    Bessoth, FG
    deMello, AJ
    Manz, A
    [J]. ANALYTICAL COMMUNICATIONS, 1999, 36 (06): : 213 - 215
  • [3] Generation of complex concentration profiles in microchannels in a logarithmically small number of steps
    Campbell, Kyle
    Groisman, Alex
    [J]. LAB ON A CHIP, 2007, 7 (02) : 264 - 272
  • [4] Fabrication of the PDMS microchip for serially diluting sample with buffer
    Chang, JK
    Bang, H
    Park, SJ
    Chung, S
    Chung, C
    Han, DC
    [J]. MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS, 2003, 9 (08): : 555 - 558
  • [5] Generation of gradients having complex shapes using microfluidic networks
    Dertinger, SKW
    Chiu, DT
    Jeon, NL
    Whitesides, GM
    [J]. ANALYTICAL CHEMISTRY, 2001, 73 (06) : 1240 - 1246
  • [6] Microfluidic diffusion diluter: bulging of PDMS microchannels under pressure-driven flow
    Holden, MA
    Kumar, S
    Beskok, A
    Cremer, PS
    [J]. JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2003, 13 (03) : 412 - 418
  • [7] Generating fixed concentration arrays in a microfluidic device
    Holden, MA
    Kumar, S
    Castellana, ET
    Beskok, A
    Cremer, PS
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2003, 92 (1-2) : 199 - 207
  • [8] A novel in-plane passive microfluidic mixer with modified Tesla structures
    Hong, CC
    Choi, JW
    Ahn, CH
    [J]. LAB ON A CHIP, 2004, 4 (02): : 109 - 113
  • [9] Generation of solution and surface gradients using microfluidic systems
    Jeon, NL
    Dertinger, SKW
    Chiu, DT
    Choi, IS
    Stroock, AD
    Whitesides, GM
    [J]. LANGMUIR, 2000, 16 (22) : 8311 - 8316
  • [10] Microfabrication inside capillaries using multiphase laminar flow patterning
    Kenis, PJA
    Ismagilov, RF
    Whitesides, GM
    [J]. SCIENCE, 1999, 285 (5424) : 83 - 85