Increased Plasma Circulating Cell-Free DNA Could Be a Potential Marker for Oral Cancer

被引:63
作者
Lin, Li-Han [1 ]
Chang, Kuo-Wei [2 ]
Kao, Shou-Yen [2 ]
Cheng, Hui-Wen [1 ]
Liu, Chung-Ji [3 ]
机构
[1] MacKay Mem Hosp, Dept Med Res, Taipei 10449, Taiwan
[2] Natl Yang Ming Univ, Dept Stomatol Med Educ & Res, Vet Gen Hosp, Inst Oral Biol,Sch Dent, Taipei 11217, Taiwan
[3] Natl Yang Ming Univ, Sch Dent, Inst Oral Biol, Dept Oral & Maxillofacial Surg,Taipei MacKay Mem, Taipei 10449, Taiwan
关键词
cell free DNA; marker; mouth neoplasm; plasma; survival; TUMOR DNA; PROGNOSTIC MARKER; LUNG-CANCER; FETAL DNA; CARCINOMA; BIOMARKER; MICRORNA; ASSOCIATION; DIAGNOSIS; BLOOD;
D O I
10.3390/ijms19113303
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Oral squamous cell carcinoma (OSCC) is a disease that affects patients worldwide. DNA of dead cells is released into the blood stream and may be isolated from plasma or serum samples. This DNA is termed cell-free DNA (cfDNA). cfDNA is increased in several types of malignancies. We investigated if there was a correlation between cfDNA levels and the progression of OSCC. Methods: Using quantitative spectrometry, we measured plasma cfDNA in 121 patients with OSCC and 50 matched controls. Mann Whitney and Wilcoxon tests were used to compare differences among various clinical variants. Receiver operating characteristic (ROC) analysis was used to obtain levels suitable for the separation of the clinical subsets. Kaplan-Meier analysis was used to assess correlation with survival. Results: Plasma cfDNA was significantly elevated in patients with OSCC relative to controls. Plasma cfDNA levels correlated with larger tumor size, cervical lymph node metastasis and late stage. Higher plasma cfDNA levels were associated with a poor prognosis of OSCC, which is a new finding. Conclusion: Plasma cfDNA could serve as a novel and easily accessible biomarker in OSCC, providing diagnostic and prognostic value.
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页数:13
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