Proteins associated with cork formation in Quercus suber L. stem tissues

被引:27
作者
Ricardo, Candido P. P. [1 ,2 ]
Martins, Isabel [1 ]
Francisco, Rita [1 ]
Sergeant, Kjell [3 ]
Pinheiro, Carla [1 ]
Campos, Alexandre [1 ]
Renaut, Jenny [3 ]
Fevereiro, Pedro [1 ,4 ]
机构
[1] UNL, ITQB, EAN, P-2780157 Oeiras, Portugal
[2] Univ Tecn Lisboa, Inst Super Agron, P-1349017 Lisbon, Portugal
[3] Ctr Rech Publ Gabriel Lippmann, Dept Environm & Agrobiotechnol, L-4422 Belvaux, Luxembourg
[4] Univ Lisbon, Fac Ciencias, P-1749016 Lisbon, Portugal
关键词
Cork; Phellem formation; Suberisation; Proteomics; Quercus suber stem; NUCLEOSIDE DIPHOSPHATE KINASE; SOLANUM-TUBEROSUM L; CYCLOPHILIN GENE; BINDING PROTEIN; PLANTS; SUBERIZATION; STRESS; IDENTIFICATION; ARABIDOPSIS; VARIABILITY;
D O I
10.1016/j.jprot.2011.02.003
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cork (phellem) formation in Quercus suber stem was studied by proteomic analysis of young shoots of increasing age (Y0, Y1 and Y4) and recently-formed phellem (Y8Ph) and xylem (Y8X) from an 8-year-old branch. In this study 99 proteins were identified, 45 excised from Y8X and 54 from Y8Ph. These ones, specifically associated with phellem, are of "carbohydrate metabolism" (28%), "defence" (22%), "protein folding, stability and degradation" (19%), "regulation/signalling" (11%), "secondary metabolism" (9%), "energy metabolism" (6%), and "membrane transport" (2%). The identification in phellem of galactosidases, xylosidases, apiose/xylose synthase, laccases and diphenol oxidases suggests intense cell wall reorganization, possibly with participation of hemicellulose/pectin biosynthesis and phenol oxidation. The identification of proteasome subunits, heat shock proteins, cyclophylin, subtilisin-like proteases, 14-3-3 proteins, Rab2 protein and enzymes interacting with nucleosides/nucleic acids gives additional evidence for cellular reorganization, involving cellular secretion, protein turnover regulation and active control processes. The high involvement in phellem of defence proteins (thioredoxin-dependent peroxidase, glutathione-S-transferase, SGT1 protein, cystatin, and chitinases) suggests a strong need for cell protection from the intense stressful events occurring in active phellem, namely, desiccation, pests/disease protection, detoxification and cell death. Identically, highly enhanced defence functions were previously reported for potato periderm formation. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:1266 / 1278
页数:13
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